Institute of Bioengineering and Nanotechnology, A*STAR, Singapore.
Chem Soc Rev. 2020 Nov 7;49(21):7602-7626. doi: 10.1039/d0cs00457j. Epub 2020 Oct 5.
Recent advancements in the creation of artificial extra base pairs (unnatural base pairs, UBPs) are opening the door to a new research area, xenobiology, and genetic alphabet expansion technologies. UBPs that function as third base pairs in replication, transcription, and/or translation enable the site-specific incorporation of novel components into DNA, RNA, and proteins. Here, we describe the UBPs developed by three research teams and their application in PCR-based diagnostics, high-affinity DNA aptamer generation, site-specific labeling of RNAs, semi-synthetic organism creation, and unnatural-amino-acid-containing protein synthesis.
最近在人工额外碱基对（非天然碱基对，UBP）的创造方面的进展为一个新的研究领域——异源生物学和遗传字母扩展技术——打开了大门。在复制、转录和/或翻译中充当第三碱基对的 UBP 能够将新的成分特异性地掺入 DNA、RNA 和蛋白质中。在这里，我们描述了三个研究团队开发的 UBP 及其在基于 PCR 的诊断、高亲和力 DNA 适体生成、RNA 的特异性标记、半合成生物体的创建和含非天然氨基酸的蛋白质合成中的应用。
