Dep. of Agronomy and Plant Genetics, Univ. of Minnesota, Saint Paul, MN, 55108.
Bayer Crop Science, 704 12th Street, Kalona, IA, 52247.
Plant Genome. 2019 Nov;12(3):1-13. doi: 10.3835/plantgenome2019.01.0007.
'Fiskeby III' harbors a combination of abiotic stress traits, including iron deficiency chlorosis (IDC) tolerance. An IDC quantitative trait locus on chromosome Gm05 was identified in genome-wide association studies and biparental populations. Fine-mapping resolved a 137-kb interval containing strong candidate genes. Iron deficiency chlorosis (IDC) is an important nutrient stress for soybean [Glycine max (L.) Merr.] grown in high-pH soils. Despite numerous agronomic attempts to alleviate IDC, genetic tolerance remains the most effective preventative measure against symptoms. In this study, two association mapping populations and a biparental mapping population were used for genetic mapping of IDC tolerance. Quantitative trait loci (QTLs) were identified on chromosomes Gm03, Gm05, and Gm06. Heterogenous inbred families were developed to fine-map the Gm05 QTL, which was uniquely supported in all three mapping populations. Fine-mapping resulted in a QTL with an interval size of 137 kb on the end of the short arm of Gm05, which produced up to a 1.5-point reduction in IDC severity on a 1 to 9 scale in near isogenic lines.
“Fiskeby III”具有多种非生物胁迫特性，包括缺铁性黄化（IDC）耐受性。在全基因组关联研究和双亲群体中鉴定到了 IDC 数量性状位点（QTL）位于染色体 Gm05 上。精细定位确定了一个包含强候选基因的 137kb 间隔。缺铁性黄化（IDC）是在高 pH 土壤中生长的大豆 [Glycine max（L.）Merr.] 的重要营养胁迫。尽管在缓解 IDC 方面进行了许多农业尝试，但遗传耐受性仍然是预防症状的最有效措施。在这项研究中，使用两个关联作图群体和一个双亲作图群体对 IDC 耐受性进行遗传作图。在染色体 Gm03、Gm05 和 Gm06 上鉴定到了数量性状位点（QTL）。杂种自交系被开发用于 Gm05 QTL 的精细作图，该 QTL 在所有三个作图群体中都得到了独特的支持。精细作图导致 Gm05 短臂末端的 QTL 间隔大小为 137kb，在近等基因系中，IDC 严重程度的评分从 1 到 9 降低了 1.5 点。
