Lin Chia-Hua, Chen Yi-Chun, Huang Pin-I
Department of Biotechnology, National Formosa University, Yunlin, 63208, Taiwan.
Nanomaterials (Basel). 2020 Oct 1;10(10):1957. doi: 10.3390/nano10101957.
The present study aimed to develop a multifunctional nanoparticle platform with properties that are beneficial in imaging, targeting, and synergistic cancer phototherapy. To this end, we synthesized novel nanoparticles composed of polydopamine, nano zero-valent iron (nZVI), and reduced graphene oxide (rGO). We immobilized nZVI on the surface of GO (nZVI/GO), then further modified nZVI/GO with dopamine to form polydopamine-conjugated nZVI/rGO (nZVI/rGO@pDA). Because nZVI/rGO@pDA absorbs near infrared radiation (NIR) and binds biomolecules of cancer cells, this platform is highly efficacious in photothermal and photodynamic cancer therapy and enables specific targeting of breast cancer cells. Use of nZVI/rGO@pDA at a low concentration (10 μg/mL) resulted in irreversible damage to MCF-7 cells under NIR irradiation (808 nm) without inducing cytotoxic effects in normal cells. Furthermore, nZVI/rGO@pDA showed high sensitivity in magnetic resonance imaging (MRI), comparable to nZVI@pDA, even at low concentration. Monitoring the treatment response through evaluation of MRI signal intensity of nZVI/rGO@pDA in phototherapeutic therapy revealed that the novel material combines the advantages of nZVI, rGO, and pDA to provide specific targeting capabilities, excellent biocompatibility, and cancer phototherapeutic and tumor imaging abilities. Thus, this platform offers great potential in terms of imaging and therapeutic effects in phototherapy treatment for breast cancer.
本研究旨在开发一种多功能纳米颗粒平台，其特性有利于成像、靶向和协同癌症光疗。为此，我们合成了由聚多巴胺、纳米零价铁（nZVI）和还原氧化石墨烯（rGO）组成的新型纳米颗粒。我们将nZVI固定在GO表面（nZVI/GO），然后用多巴胺进一步修饰nZVI/GO，形成聚多巴胺共轭的nZVI/rGO（nZVI/rGO@pDA）。由于nZVI/rGO@pDA吸收近红外辐射（NIR）并结合癌细胞的生物分子，该平台在光热和光动力癌症治疗中非常有效，并能够特异性靶向乳腺癌细胞。在低浓度（10μg/mL）下使用nZVI/rGO@pDA在近红外辐射（808nm）下对MCF-7细胞造成不可逆损伤，而不会对正常细胞产生细胞毒性作用。此外，nZVI/rGO@pDA在磁共振成像（MRI）中表现出高灵敏度，即使在低浓度下也与nZVI@pDA相当。通过评估光疗中nZVI/rGO@pDA的MRI信号强度来监测治疗反应，结果表明这种新型材料结合了nZVI、rGO和pDA的优点，提供了特异性靶向能力、优异的生物相容性以及癌症光疗和肿瘤成像能力。因此，该平台在乳腺癌光疗的成像和治疗效果方面具有巨大潜力。
