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氢氧化铝纳米颗粒与包裹保护性抗原结构域4的非离子表面活性剂囊泡联合给药显示出增强的免疫反应及对炭疽的卓越保护作用。

Co-Administration of Aluminium Hydroxide Nanoparticles and Protective Antigen Domain 4 Encapsulated Non-Ionic Surfactant Vesicles Show Enhanced Immune Response and Superior Protection against Anthrax.

作者信息

Gogoi Himanshu, Mani Rajesh, Malik Anshu, Sehrawat Parveen, Bhatnagar Rakesh

机构信息

Laboratory of Molecular Biology and Genetic Engineering, School of Biotechnology, Jawaharlal Nehru University, New Delhi 110067, India.

VC Office, Banaras Hindu University, Varanasi 221005, India.

出版信息

Vaccines (Basel). 2020 Oct 1;8(4):571. doi: 10.3390/vaccines8040571.

DOI:10.3390/vaccines8040571
PMID:33019545
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7711981/
Abstract

Aluminium salts have been the adjuvant of choice in more than 100 licensed vaccines. Here, we have studied the synergistic effect of aluminium hydroxide nanoparticles (AH np) and non-ionic surfactant-based vesicles (NISV) in modulating the immune response against protective antigen domain 4 (D4) of . NISV was prepared from Span 60 and cholesterol, while AH np was prepared from aluminium chloride and sodium hydroxide. AH np was co-administered with NISV encapsulating D4 (NISV-D4) to formulate AHnp/NISV-D4. The antigen-specific immune response of AHnp/NISV-D4 was compared with that of commercial alhydrogel (alhy) co-administered with NISV-D4 (alhydrogel/NISV-D4), NISV-D4, AHnp/D4, and alhydrogel/D4. Co-administration of NISV-D4 with AH np greatly improved the D4-specific antibody titer as compared to the control groups. Based on IgG isotyping and ex vivo cytokine analysis, AHnp/NISV-D4 generated a balanced Th1/Th2 response. Furthermore, AH np/NISV-D4 showed superior protection against anthrax spore challenge in comparison to other groups. Thus, we demonstrate the possibility of developing a novel combinatorial nanoformulation capable of augmenting both humoral and cellular response, paving the way for adjuvant research.

摘要

铝盐一直是100多种已获许可疫苗中首选的佐剂。在此，我们研究了氢氧化铝纳米颗粒（AH np）和非离子表面活性剂基囊泡（NISV）在调节针对[某种抗原]保护性抗原结构域4（D4）的免疫反应中的协同作用。NISV由司盘60和胆固醇制备，而AH np由氯化铝和氢氧化钠制备。AH np与包裹D4的NISV（NISV-D4）共同给药，以配制AHnp/NISV-D4。将AHnp/NISV-D4的抗原特异性免疫反应与与NISV-D4共同给药的商业氢氧化铝凝胶（alhy）（alhydrogel/NISV-D4）、NISV-D4、AHnp/D4和alhydrogel/D4的抗原特异性免疫反应进行比较。与对照组相比，NISV-D4与AH np共同给药大大提高了D4特异性抗体滴度。基于IgG亚型分析和体外细胞因子分析，AHnp/NISV-D4产生了平衡的Th1/Th2反应。此外，与其他组相比，AH np/NISV-D4在抵抗炭疽芽孢攻击方面表现出更好的保护作用。因此，我们证明了开发一种能够增强体液和细胞反应的新型组合纳米制剂的可能性，为佐剂研究铺平了道路。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84b9/7711981/8899609e3429/vaccines-08-00571-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84b9/7711981/4f825225da9f/vaccines-08-00571-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84b9/7711981/747076491696/vaccines-08-00571-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84b9/7711981/d22d3cfdbcf2/vaccines-08-00571-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84b9/7711981/385418b7f5b1/vaccines-08-00571-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84b9/7711981/8899609e3429/vaccines-08-00571-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84b9/7711981/4f825225da9f/vaccines-08-00571-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84b9/7711981/747076491696/vaccines-08-00571-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84b9/7711981/d22d3cfdbcf2/vaccines-08-00571-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84b9/7711981/385418b7f5b1/vaccines-08-00571-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84b9/7711981/8899609e3429/vaccines-08-00571-g005.jpg

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