Ramos Lívia S, Silva Laura N, Branquinha Marta H, Santos André L S
Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes (LEAMER), Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes (IMPG), Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro 21941-901, Brazil.
Programa de Pós-Graduação em Bioquímica (PPGBq), Instituto de Química (IQ), Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro 21941-909, Brazil.
J Fungi (Basel). 2020 Oct 1;6(4):201. doi: 10.3390/jof6040201.
complex (, and var. ) is well-known for its resistance profile to different available antifungal drugs. Although echinocandins are the most effective class of antifungal compounds against the species complex, clinical isolates resistant to caspofungin, micafungin and anidulafungin have already been reported. In this work, we present a literature review regarding the effects of echinocandins on this emergent fungal complex. Published data has revealed that micafungin and anidulafungin were more effective than caspofungin against the species forming the complex. Subsequently, we investigated the susceptibilities of both planktonic and biofilm forms of 12 Brazilian clinical isolates of the complex towards caspofungin and micafungin (anidulafungin was unavailable). The planktonic cells of all the fungal isolates were susceptible to both of the test echinocandins. Interestingly, echinocandins caused a significant reduction in the biofilm metabolic activity (viability) of almost all fungal isolates (11/12, 91.7%). Generally, the biofilm biomasses were also affected (reduction range 20-60%) upon exposure to caspofungin and micafungin. This is the first report of the anti-biofilm action of echinocandins against the multidrug-resistant opportunistic pathogens comprising the complex, and unveils the therapeutic potential of these compounds.
复合体（，以及变种 ）以其对不同现有抗真菌药物的耐药谱而闻名。尽管棘白菌素是针对该复合体最有效的一类抗真菌化合物，但对卡泊芬净、米卡芬净和阿尼芬净耐药的临床分离株已被报道。在这项工作中，我们对棘白菌素对这种新出现的真菌复合体的作用进行了文献综述。已发表的数据表明，米卡芬净和阿尼芬净对构成该复合体的菌种比卡泊芬净更有效。随后，我们研究了巴西12株该复合体临床分离株的浮游菌和生物被膜形式对卡泊芬净和米卡芬净（阿尼芬净无法获得）的敏感性。所有真菌分离株的浮游细胞对两种受试棘白菌素均敏感。有趣的是，棘白菌素使几乎所有真菌分离株（11/12，91.7%）的生物被膜代谢活性（活力）显著降低。一般来说，在接触卡泊芬净和米卡芬净后，生物被膜生物量也受到影响（降低范围为20 - 60%）。这是关于棘白菌素对构成该复合体的多重耐药机会性病原体的抗生物被膜作用的首次报道，并揭示了这些化合物的治疗潜力。
