Laboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Department of Anatomy, Histology and Physiology, Faculty of Animal Science and Veterinary Medicine, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh.
J Biomed Mater Res A. 2021 Jul;109(7):1220-1231. doi: 10.1002/jbm.a.37115. Epub 2020 Oct 15.
We investigated spatiotemporal changes in host tissues during foreign body reactions. Silicone tube was subcutaneously embedded into ICR mice, and tissue surrounding silicone (TSS) was observed at 2, 7, 14, 21, 28, 43, and 70 days (D) postsurgery. The thin layer (TL) and loose connective tissues (LCTs) (inside and outside the TSS) developed until D21 and densified afterward. Neutrophils infiltrated the TSS until D14 and formed neutrophil extracellular traps (NETs) in the TL during D7-21. In the LCTs, mast cell counts increased until D21, and macrophage numbers peaked at D14. Several macrophages showed LYVE-1 expression, supporting a tissue-remodeling role. Developmental indices of collagen fibers (CFs) and reticular fibers (RFs) increased during D2-21. NETs, but not neutrophils, were detected after D28. Mast cell numbers peaked at D43 and were maintained until D70. Myofibroblasts consistently localized to the TL from D14. During D21-28, the area of connective tissue (CNT), and CFs and RFs decreased and increased, respectively, and both remained constant during D28-70. The CF density remained constant from D21 and increased at D70. Thus, TSS showed two phases: inflammation and CNT development (D2-21), and inflammation convergence and CNT stabilization (D28-70). These results provide insights into foreign body reactions in clinical cases.
我们研究了异物反应过程中宿主组织的时空变化。将硅胶管皮下植入 ICR 小鼠，在术后 2、7、14、21、28、43 和 70 天观察硅胶周围组织（TSS）。薄层层（TL）和疏松结缔组织（LCT）（TSS 内外）在 D21 之前发育，并在此后变得致密。中性粒细胞在 TSS 中浸润直到 D14，并在 D7-21 期间在 TL 中形成中性粒细胞胞外陷阱（NETs）。在 LCT 中，肥大细胞计数直到 D21 增加，而巨噬细胞数量在 D14 达到峰值。一些巨噬细胞表达 LYVE-1，支持其组织重塑作用。胶原纤维（CFs）和网状纤维（RFs）的发育指数在 D2-21 期间增加。D28 后检测到 NETs，但未检测到中性粒细胞。肥大细胞数量在 D43 达到峰值并维持到 D70。肌成纤维细胞从 D14 开始一直定位于 TL。在 D21-28 期间，结缔组织（CNT）、CFs 和 RFs 的面积分别减少和增加，在 D28-70 期间保持不变。CF 密度从 D21 开始保持不变，并在 D70 增加。因此，TSS 显示出两个阶段：炎症和 CNT 发育（D2-21），以及炎症收敛和 CNT 稳定（D28-70）。这些结果为临床病例中的异物反应提供了深入了解。
