Department of Anatomy and Comparative Pathology and Toxicology, University of Cordoba, Cordoba, Spain.
CICAP - Food Research Center, Córdoba, Spain.
Vet Rec. 2020 Nov 28;187(11):e101. doi: 10.1136/vr.105945. Epub 2020 Oct 6.
is a foodborne bacterial pathogen that causes listeriosis, an infectious disease in animals and people, with pigs acting as asymptomatic reservoirs. In August 2019 an outbreak associated with the consumption of pork meat caused 222 human cases of listeriosis in Spain. Determining the diversity as well as the virulence potential of strains from pigs is important to public health.
The behaviour of 23 strains recovered from pig tonsils, meat and skin was compared by studying (1) internalin A, internalin B, listeriolysin O, actin assembly-inducing protein and PrfA expression levels, and (2) their invasion and intracellular growth in eukaryotic cells.
Marked differences were found in the expression of the selected virulence factors and the invasion and intracellular replication phenotypes of strains. Strains obtained from meat samples and belonging to serotype 1/2a did not have internalin A anchored to the peptidoglycan. Some strains expressed higher levels of the studied virulence factors and invaded and replicated intracellularly more efficiently than an epidemic reference strain (F2365).
This study demonstrates the presence of virulent strains with virulent potential in pigs, with valuable implications in veterinary medicine and food safety.
是一种食源性细菌病原体，可导致李斯特菌病，这是一种动物和人类的传染病，猪作为无症状的储主。2019 年 8 月，一起与食用猪肉有关的疫情在西班牙导致了 222 例人类李斯特菌病病例。确定猪源菌株的多样性及其毒力潜能对公共卫生很重要。
通过研究（1）内化素 A、内化素 B、李斯特菌溶血素 O、肌动蛋白组装诱导蛋白和 PrfA 的表达水平，以及（2）它们在真核细胞中的入侵和细胞内生长，比较了从猪扁桃体、肉和皮肤中回收的 23 株菌的行为。
在所选择的毒力因子的表达和菌株的入侵和细胞内复制表型方面发现了明显的差异。从肉样中获得的属于 1/2a 血清型的菌株没有锚定在肽聚糖上的内化素 A。一些菌株表达了更高水平的研究毒力因子，并且比流行的参考菌株（F2365）更有效地入侵和复制细胞内。
本研究表明猪中存在具有潜在毒力的毒力菌株，这对兽医和食品安全具有重要意义。
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