从绵羊和山羊养殖场的散装罐牛奶中分离——从临床工作到生物信息学研究：患病率、与牛奶质量的关联、抗生素敏感性、预测因素、全基因组序列和系统发育关系

Isolation of from Bulk-Tank Milk of Sheep and Goat Farms-From Clinical Work to Bioinformatics Studies: Prevalence, Association with Milk Quality, Antibiotic Susceptibility, Predictors, Whole Genome Sequence and Phylogenetic Relationships.

作者信息

Lianou Daphne T, Skoulakis Anargyros, Michael Charalambia K, Katsarou Eleni I, Chatzopoulos Dimitris C, Solomakos Nikolaos, Tsilipounidaki Katerina, Florou Zoe, Cripps Peter J, Katsafadou Angeliki I, Vasileiou Natalia G C, Dimoveli Konstantina S, Bourganou Maria V, Liagka Dimitra V, Papatsiros Vasileios G, Kontou Panagiota I, Mavrogianni Vasia S, Caroprese Mariangela, Petinaki Efthymia, Fthenakis George C

机构信息

Veterinary Faculty, University of Thessaly, 43100 Karditsa, Greece.

Institute for Bioinnovation, BSRC Alexander Fleming, 16672 Vari, Greece.

出版信息

Biology (Basel). 2022 Jun 6;11(6):871. doi: 10.3390/biology11060871.

DOI:10.3390/biology11060871

PMID:35741392

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9220212/

Abstract

A cross-sectional study was performed in 325 sheep and 119 goat dairy farms in Greece. Samples of bulk-tank milk were examined by standard microbiological techniques for spp. was isolated from one (0.3%) and from three (0.9%) sheep farms. No associations between the isolation of or and milk quality were found. No resistance to antibiotics was identified. Three variables emerged as significant predictors of isolation of the organism: the presence of pigs, low average relative humidity and a high number of ewes on the farm. The three isolates were assessed in silico for identification of plasmids, prophages, antibiotic resistance genes, virulence factors, CRISPRs and CAS genes. Phylogenetic analysis using the core genome revealed that the three strains belonged to the subsp. branch and were especially close to the PAM 55 strain. All strains of the branch appeared to be very similar, with the distance between them being small.

摘要

在希腊的325个绵羊奶场和119个山羊奶场进行了一项横断面研究。采用标准微生物技术对大容量罐奶样本进行检测，以检测特定菌种。在一个（0.3%）绵羊奶场分离出了[某种菌]，在三个（0.9%）绵羊奶场分离出了[另一种菌]。未发现[某种菌]或[另一种菌]的分离与牛奶质量之间存在关联。未发现对抗生素的耐药性。有三个变量成为该生物体分离的显著预测因素：猪场的存在、低平均相对湿度以及奶场上大量的母羊。对这三株分离株进行了计算机模拟评估，以鉴定质粒、原噬菌体、抗生素耐药基因、毒力因子、CRISPRs和CAS基因。使用核心基因组进行的系统发育分析表明，这三株菌株属于[某种亚种]分支，并且特别接近PAM 55菌株。该分支的所有菌株似乎非常相似，它们之间的距离很小。