Nishiwaki K, Hayashi N, Irie S, Chung D H, Harashima S, Oshima Y
Mol Gen Genet. 1987 Jun;208(1-2):159-67. doi: 10.1007/BF00330437.
The nucleotide sequence of a 2.1 kb DNA fragment bearing the HIS5 gene of Saccharomyces cerevisiae, which encodes histidinol-phosphate aminotransferase (EC 2.6.1.9), has been determined. An open reading frame of 1,152 bp was found. S1 nuclease mapping indicated that the major transcription starts at position -37 from the ATG codon and the minor (approximately 20%) at -34 in both repressive and derepressive conditions. Northern analysis indicated that transcription of the HIS5 gene is under the general control of amino acid biosynthesis. The 5' noncoding region of the gene, thus far examined up to position -616, contains three copies of sequences homologous to the short repeats of the consensus sequence, 5'-AATGTGACTC-3', suggested for general amino acid control in the HIS1, HIS3, HIS4, and TRP5 at positions -336, -275 and -205. The consensus sequence closest to the open reading frame was shown to be necessary but not sufficient for general amino acid control, by examination of beta-galactosidase appearance in S. cerevisiae cells carrying various mutant HIS5 promoter regions fused to the lac'Z gene and inserted at the leu2 locus of chromosome III.
已确定携带酿酒酵母HIS5基因的一个2.1 kb DNA片段的核苷酸序列，该基因编码组氨醇磷酸氨基转移酶（EC 2.6.1.9）。发现了一个1152 bp的开放阅读框。S1核酸酶图谱分析表明，在阻遏和去阻遏条件下，主要转录起始于距ATG密码子-37位，次要转录起始于（约20%）-34位。Northern分析表明，HIS5基因的转录受氨基酸生物合成的总体调控。该基因的5'非编码区，迄今为止检测到-616位，在-336、-275和-205位含有三个与共有序列5'-AATGTGACTC-3'的短重复序列同源的序列拷贝，该共有序列被认为参与HIS1、HIS3、HIS4和TRP5的一般氨基酸调控。通过检测携带与lac'Z基因融合并插入到III号染色体leu2位点的各种突变HIS5启动子区域的酿酒酵母细胞中β-半乳糖苷酶的出现情况，发现最接近开放阅读框处的共有序列对于一般氨基酸调控是必要的，但并不充分。
