The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China; VIP Center, Hangzhou Dental Hospital, Hangzhou, China.
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
J Dent. 2018 Sep;76:24-31. doi: 10.1016/j.jdent.2018.04.013. Epub 2018 Apr 18.
To evaluate the effect of naringenin on the biofilm formation of Streptococcus mutans (S. mutans), and to investigate its mechanisms of action and biological toxicity.
Minimum inhibitory concentrations, growth curves, and biofilm inhibition rates of naringenin were determined to assess its antimicrobial effect on S. mutans. The morphology of S. mutans and the structure of biofilm were observed by FESEM and CLSM. Bacterial aggregation, bacterial surface hydrophobicity, and real-time PCR for gtfB, gtfC, comD, comE, and luxS mRNA expression were assessed to preliminarily investigate the mechanisms of action. MTT test using human dental pulp cells (HDPCs) was also performed to investigate cytotoxicity.
The S.mutans growth curves, FESEM, CLSM showed that both 100 and 200 μg/mL of naringenin obviously inhibited S. mutans growth and biofilm formation, increased S. mutans surface hydrophobicity, reduced bacterial aggregation, and downregulated the mRNA expression of gtfB, gtfC, comD, comE, and luxS. However, naringenin at 200 μg/mL slightly decreased the growths of HDPCs compared with 100 μg/mL.
Naringenin at 100 and 200 μg/mL suppressed the second (bacterial adhesion) and third stages (biofilm maturation) of S. mutans biofilm formation.
Naringenin is promising for dental clinic promotion to prevent the biofilm formation of S. mutans, serving as a safe anti-caries agent at an appropriate concentration.
评估柚皮素对变形链球菌（S. mutans）生物膜形成的影响，并探讨其作用机制和生物毒性。
通过测定柚皮素对 S. mutans 的最小抑菌浓度、生长曲线和生物膜抑制率，评估其对 S. mutans 的抗菌作用。采用 FESEM 和 CLSM 观察 S. mutans 的形态和生物膜结构。通过细菌聚集、细菌表面疏水性和实时 PCR 检测 gtfB、gtfC、comD、comE 和 luxS mRNA 表达，初步探讨其作用机制。采用人牙髓细胞（HDPCs）MTT 试验研究细胞毒性。
S.mutans 生长曲线、FESEM、CLSM 结果表明，100 和 200μg/ml 的柚皮素明显抑制 S. mutans 的生长和生物膜形成，增加 S. mutans 表面疏水性，减少细菌聚集，并下调 gtfB、gtfC、comD、comE 和 luxS 的 mRNA 表达。然而，与 100μg/ml 相比，200μg/ml 的柚皮素略微降低了 HDPCs 的生长。
100 和 200μg/ml 的柚皮素抑制了 S. mutans 生物膜形成的第二阶段（细菌黏附）和第三阶段（生物膜成熟）。
柚皮素有望在牙科临床推广，以防止 S. mutans 生物膜的形成，作为一种安全的抗龋剂，在适当的浓度下使用。
