Derouich Mgal, Bouhlali Eimad Dine Tariq, Bammou Mohamed, Hmidani Abdelbasset, Sellam Khalid, Alem Chakib
Biochemistry of Natural Products, Faculty of Sciences and Techniques, Moulay Ismail University, 52000 Errachidia, Morocco.
Biology, Environment and Health Team, Faculty of Sciences and Techniques, Moulay Ismail University, 52000 Errachidia, Morocco.
Scientifica (Cairo). 2020 Sep 22;2020:3971041. doi: 10.1155/2020/3971041. eCollection 2020.
For a long time, species have been widely employed in the southeast of Morocco for culinary and folk healing purposes. In the current study, we investigated three herbs known as coriander (), celery (), and parsley () for their antioxidant, antiperoxidative, and antihemolytic properties. The HPLC-DAD has been used to classify and measure phenolic compounds. The major phenolic compounds studied were p-coumaric, chlorogenic, caffeic acids, luteolin, and quercetin. The polyphenol level was also estimated via Folin-Ciocalteu's method, aluminium chloride, and acidified vanillin. Parsley showed the highest polyphenol level and, thus, showed potential antioxidant activities demonstrated by DPPH, ABTS scavenging tests, and reducing power (FRAP), as well as TBARS assays. Very strong correlations were depicted among phenol levels and antioxidant assays ( ≥ 0.910) and among antihemolytic activity and flavonoids ( ≥ 0.927), indicating the implication of phenolic compounds, mainly flavonoids, in the antiradical properties. These finding may prove the traditional use of these species in the management of numerous disorders cited within the Moroccan pharmacopoeia.
长期以来,摩洛哥东南部的一些植物物种被广泛用于烹饪和民间治疗。在本研究中,我们研究了三种草药,即香菜、芹菜和欧芹的抗氧化、抗过氧化和抗溶血特性。采用高效液相色谱 - 二极管阵列检测法(HPLC - DAD)对酚类化合物进行分类和测定。所研究的主要酚类化合物有对香豆酸、绿原酸、咖啡酸、木犀草素和槲皮素。还通过福林 - 西奥尔特法、氯化铝和酸化香草醛法估算了多酚含量。欧芹显示出最高的多酚含量,因此通过二苯基苦味酰基自由基(DPPH)、2,2'-联氮 - 双 - 3 - 乙基苯并噻唑啉 - 6 - 磺酸(ABTS)清除试验、还原能力(铁离子还原抗氧化能力,FRAP)以及硫代巴比妥酸反应物(TBARS)测定显示出潜在的抗氧化活性。酚类含量与抗氧化试验之间(r≥0.910)以及抗溶血活性与黄酮类化合物之间(r≥0.927)呈现出非常强的相关性,表明酚类化合物,主要是黄酮类化合物,在抗自由基特性中起作用。这些发现可能证明了这些植物物种在摩洛哥药典中所提及的多种疾病治疗中的传统用途。