Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, 60131, Ancona, Italy.
Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, 60131, Ancona, Italy; Area de Nutrición y Salud, Universidad Internacional Iberoamericana (UNINI), Campeche, C.P.24040, Mexico.
Food Chem Toxicol. 2018 Apr;114:128-140. doi: 10.1016/j.fct.2018.02.038. Epub 2018 Feb 16.
A protracted pro-inflammatory state is the common denominator in the development, progression and complication of the common chronic diseases. Dietary antioxidants represent an efficient tool to counteract this inflammatory state. The aim of the present work was to evaluate the effects of strawberry extracts on inflammation evoked by E. Coli lipopolysaccharide in Human Dermal Fibroblast, by measuring reactive oxygen species production, apoptosis rate, antioxidant enzymes activity, mitochondria functionality and also investigating the molecular pathway involved in inflammatory and antioxidant response. The results demonstrated that strawberry pre-treatment reduced intracellular reactive oxygen species levels, apoptotic rate, improved antioxidant defences and mitochondria functionality in lipopolysaccharide -treated cells. Strawberry exerted these protective activities through the inhibition of the NF-kB signalling pathway and the stimulation of the Nrf2 pathway, with a mechanism AMPK-dependent. These results confirm the health benefits of strawberry in the prevention of inflammation and oxidative stress condition in lipopolysaccharide-treated cells.
长期的促炎状态是常见慢性疾病的发展、进展和并发症的共同特征。膳食抗氧化剂是对抗这种炎症状态的有效工具。本研究的目的是评估草莓提取物对人真皮成纤维细胞中大肠杆菌脂多糖诱导的炎症的影响，通过测量活性氧（ROS）的产生、细胞凋亡率、抗氧化酶活性、线粒体功能，并研究参与炎症和抗氧化反应的分子途径。结果表明，草莓预处理可降低脂多糖处理细胞中的细胞内活性氧水平、细胞凋亡率、提高抗氧化防御和线粒体功能。草莓通过抑制 NF-kB 信号通路和刺激 Nrf2 通路来发挥这些保护作用，其机制依赖于 AMPK。这些结果证实了草莓在预防脂多糖处理细胞中的炎症和氧化应激方面的健康益处。
