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一种用于检测菌尿症的新型商用试剂盒(UROBACT)的评估。

Evaluation of a new commercial kit (UROBACT) for detection of bacteriuria.

作者信息

Christensen K K, Christensen P

出版信息

Scand J Prim Health Care. 1987 May;5(2):113-6. doi: 10.3109/02813438709013986.

DOI:10.3109/02813438709013986
PMID:3303249
Abstract

A new commercially available method (UROBACT, AB Dermaci, Sweden) for detection of bacteriuria was compared to a conventional semiquantitative bacteriological method. The kit consisted of two tubes, which were inoculated with urine in two different dilutions. Positive tests were recorded as a colour change from red to yellow in both tubes, and the test was considered negative if one or both tubes remained red. When studying 556 urine samples, the test detected 153 of 163 samples with greater than or equal to 10(5) colony forming units (CFU) of bacteria per ml, i.e. the sensitivity was 93.9%. Among the samples containing less than 10(5) CFU/ml, 347 of 357 were recorded as negative, giving a specificity of 97.2%. Although relatively few urine samples containing Staphylococcus saprophyticus and Pseudomonas pyoceanea were tested the results indicated that the method was less reliable for these species. However, compared to dip-slides, which also give poor results with S. saprophyticus, the new test has several advantages, including a more simple interpretation, i.e. a distinct colour change.

摘要

一种新的可商购的检测菌尿症的方法(UROBACT,瑞典AB Dermaci公司)与传统的半定量细菌学方法进行了比较。该试剂盒由两个试管组成,分别接种两种不同稀释度的尿液。阳性检测结果记录为两个试管中的颜色均从红色变为黄色,如果一个或两个试管仍为红色,则该检测被视为阴性。在研究556份尿液样本时,该检测方法检测出每毫升细菌菌落形成单位(CFU)大于或等于10⁵的163份样本中的153份,即灵敏度为93.9%。在每毫升含有少于10⁵CFU的样本中,357份中的347份被记录为阴性,特异性为97.2%。尽管检测的含有腐生葡萄球菌和脓绿假单胞菌的尿液样本相对较少,但结果表明该方法对这些菌种的可靠性较低。然而,与对腐生葡萄球菌检测结果也不佳的浸片法相比,这种新检测方法有几个优点,包括解释更简单,即颜色有明显变化。

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