State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, P. R. China.
South China Sea Fisheries Research Institute (CAFS), Guangzhou, PR China.
Virus Genes. 2020 Dec;56(6):749-755. doi: 10.1007/s11262-020-01800-1. Epub 2020 Oct 8.
Infectious spleen and kidney necrosis virus (ISKNV), causing serious infectious diseases to marine and freshwater fishes, is the type species of the genus Megalocytivirus, family Iridoviridae. In this study, the transcriptional programs of ISKNV in vitro (MFF-1 cells) and in vivo (spleens from mandarin fish) were investigated using real-time PCR. Transcription of all the putative open reading frames (ORFs) of ISKNV was verified. The temporal expression patterns of ISKNV ORFs in vitro and in vivo, including peak expression times (PETs) and relative maximal expression levels, were determined and compared. The K-means clustering with Spearman rank correlation was generated in heat maps constructed based on ISKNV ORF expression profiles in vivo and in vitro. The current study may provide a global picture of ISKNV infection at the transcription level and help better understand the molecular pathogenic mechanism of megalocytiviruses.
传染性脾肾坏死病毒（ISKNV）可引起海水和淡水鱼类的严重传染病，是虹彩病毒科 Megalocytivirus 属的模式种。本研究采用实时 PCR 技术，研究了 ISKNV 在体外（MFF-1 细胞）和体内（鳜鱼脾脏）的转录程序。验证了 ISKNV 所有推定开放阅读框（ORF）的转录。确定并比较了 ISKNV ORF 在体外和体内的时间表达模式，包括峰值表达时间（PET）和相对最大表达水平。基于体内和体外 ISKNV ORF 表达谱构建的热图中生成了 K-均值聚类与 Spearman 秩相关。本研究可能提供了 ISKNV 在转录水平感染的全貌，有助于更好地了解 megalocytiviruses 的分子发病机制。
