Mizuhashi Fumi, Morita Takao, Toya Shuji, Sato Ritsuko, Watarai Yuko, Koide Kaoru
Department of Removable Prosthodontics, the Nippon Dental University School of Life Dentistry at Niigata, Niigata 951-8580, Japan.
Department of Biochemistry, the Nippon Dental University School of Life Dentistry at Niigata, Niigata 951-8580, Japan.
Geriatrics (Basel). 2020 Oct 7;5(4):70. doi: 10.3390/geriatrics5040070.
Oral dryness as a side effect of certain drugs is increasing. The aim of this study was to examine the change of the protein ingredient in saliva of oral dryness patients caused by calcium blocker. Six patients taking calcium blocker and six healthy elderly were enrolled. Unstimulated salivary flow rate, protein concentration, and flow rate of protein were measured and compared between the patients taking calcium blocker and healthy elderly. iTRAQ (Isobaric Tag for Relative and Absolute Quantitation) proteomic analysis was performed to extract the salivary protein changed in patient taking calcium blocker, and the intensities of Western blotting products were quantified (unpaired -test). Unstimulated salivary flow rate was significantly lower on patients taking calcium blocker ( < 0.01). Protein concentration tended to be higher and the flow rate of protein tended to be lower on patients. As the result of iTRAQ proteomic analysis, calmodulin-like protein 3, glutathione S-transferase P, and keratin type I cytoskeletal 13 increased characteristically in patient taking calcium blocker, and the expression in calmodulin-like protein 3 was significantly larger ( < 0.01). The results of this study indicated that calmodulin-like protein 3 increased in patients taking calcium blocker and could be a salivary biomarker for oral dryness caused by calcium blocker.
作为某些药物的副作用，口腔干燥的情况日益增多。本研究旨在探讨钙通道阻滞剂所致口腔干燥患者唾液中蛋白质成分的变化。选取6例服用钙通道阻滞剂的患者和6例健康老年人作为研究对象。测量并比较服用钙通道阻滞剂的患者与健康老年人的非刺激性唾液流速、蛋白质浓度及蛋白质流速。采用iTRAQ（相对与绝对定量等压标签）蛋白质组学分析方法，提取服用钙通道阻滞剂患者唾液中发生变化的蛋白质，并对蛋白质印迹产物的强度进行定量分析（非配对t检验）。服用钙通道阻滞剂的患者非刺激性唾液流速显著降低（P<0.01）。患者的蛋白质浓度有升高趋势，而蛋白质流速有降低趋势。iTRAQ蛋白质组学分析结果显示，服用钙通道阻滞剂的患者中，类钙调蛋白3、谷胱甘肽S-转移酶P和角蛋白I型细胞骨架13呈特征性增加，且类钙调蛋白3的表达显著升高（P<0.01）。本研究结果表明，服用钙通道阻滞剂的患者类钙调蛋白3增加，可能是钙通道阻滞剂所致口腔干燥的唾液生物标志物。
