Fellag Mustapha, Gouba Nina, Bedotto Marielle, Sakana Moussa, Zingué Dezemon, Tarnagda Zékiba, Million Matthieu, Drancourt Michel
IHU Méditerranée Infection, 13005 Marseille, France.
Aix-Marseille Univ., IRD, MEPHI, IHU Méditerranée Infection, 13005 Marseille, France.
Microorganisms. 2020 Oct 7;8(10):1544. doi: 10.3390/microorganisms8101544.
causes pulmonary tuberculosis, a deadly infection of which the clinical expression and prognosis are not fully understood at the individual level, apart from genetic susceptibility traits. We investigated whether individual gut microbiota may correlate with pulmonary tuberculosis status. Culturomics investigations of gut microbiota in two pulmonary tuberculosis patients and two controls in Burkina Faso found 60 different bacterial species in patients and 97 in controls, including 45 in common. Further analysis of the results at the individual level indicated seven bacteria, including and , which were exclusively cultured in controls. Blind quantitative PCR-based exploration of faeces samples in two cohorts in Burkina Faso and in France confirmed a nonsignificant association of and with controls. Further in vitro explorations found four and strains inhibiting the growth of strains representative of four different lineages as well as , , and , in an inoculum-dependent manner. Heat-killed or were ineffective. These unprecedented observations of direct interactions between gut and with complex mycobacteria suggest that gut microbiota may modulate the expression of pulmonary tuberculosis.
导致肺结核，这是一种致命的感染，除了遗传易感性特征外，其临床表现和预后在个体层面上尚未完全了解。我们调查了个体肠道微生物群是否可能与肺结核状态相关。对布基纳法索两名肺结核患者和两名对照的肠道微生物群进行的培养组学研究发现，患者中有60种不同的细菌种类，对照中有97种，其中45种是共有的。在个体水平上对结果进行的进一步分析表明，有7种细菌，包括[细菌名称1]和[细菌名称2]，仅在对照中培养出来。在布基纳法索和法国的两个队列中，基于盲法定量PCR对粪便样本进行的探索证实，[细菌名称1]和[细菌名称2]与对照之间存在无统计学意义的关联。进一步的体外探索发现，4种[细菌名称3]和[细菌名称4]菌株以接种物依赖的方式抑制了代表4个不同谱系的[分枝杆菌名称]菌株以及[分枝杆菌名称]、[分枝杆菌名称]和[分枝杆菌名称]的生长。热灭活的[细菌名称3]或[细菌名称4]无效。肠道[细菌名称3]和[细菌名称4]与复杂分枝杆菌之间直接相互作用的这些前所未有的观察结果表明，肠道微生物群可能会调节肺结核的表达。
