Achache Wafaa, Mege Jean Louis, Fellag Mustapha, Drancourt Michel
Aix-Marseille Univ, IRD, AP-HM, MEPHI, Marseille, France.
IHU Méditerranée Infection, Marseille, France.
Access Microbiol. 2023 Jun 23;5(6). doi: 10.1099/acmi.0.000471.v3. eCollection 2023.
, a commensal intestinal bacterium, was demonstrated to inhibit the growth of some complex (MTC) species that cause tuberculosis in humans and mammals. To further explore this preliminary observation, we cross-investigated five strains and seven MTC strains representative of four MTC species using a standardized quantitative agar well diffusion assay. All five strains, calibrated at 10 MacFarland, inhibited the growth of all strains with various susceptibility profiles, but no inhibition was observed with lower inoculums. Further, eight freeze-dried cell-free culture supernatants (CFCS) inhibited the growth of , and , the most susceptible MTC species (inhibition diameter 25±1 mm), proportionally to CFCS protein concentrations. The data reported here indicate that the secretome inhibited growth of all MTC species of medical interest, which broadens previously reported data. In the gut, the secretome may modulate the expression of tuberculosis, exhibiting an anti-tuberculosis effect, with some protective roles in human and animal health.
一种肠道共生细菌被证明能抑制一些在人类和哺乳动物中引起结核病的分枝杆菌复合群（MTC）物种的生长。为了进一步探究这一初步观察结果，我们使用标准化定量琼脂孔扩散试验对代表四种MTC物种的五株[细菌名称未给出]菌株和七株MTC菌株进行了交叉研究。所有五株校准为10麦氏浊度的[细菌名称未给出]菌株，均抑制了所有具有不同药敏谱的[细菌名称未给出]菌株的生长，但接种量较低时未观察到抑制作用。此外，八种[细菌名称未给出]冻干无细胞培养上清液（CFCS）抑制了最敏感的MTC物种[细菌名称未给出]、[细菌名称未给出]和[细菌名称未给出]的生长（抑菌直径25±1毫米），且与CFCS蛋白浓度成比例。此处报告的数据表明，[细菌名称未给出]分泌组抑制了所有具有医学意义的MTC物种的生长，这拓宽了先前报告的数据。在肠道中，[细菌名称未给出]分泌组可能调节结核病的表达，表现出抗结核作用，对人类和动物健康具有一定的保护作用。
