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菜豆遗传育种的下一代测序技术展望。

Prospects of next generation sequencing in lentil breeding.

机构信息

Division of Crop Improvement, ICAR-Indian Institute of Pulses Research, Kalyanpur, Kanpur, 208024, India.

出版信息

Mol Biol Rep. 2020 Nov;47(11):9043-9053. doi: 10.1007/s11033-020-05891-9. Epub 2020 Oct 10.

Abstract

Lentil is an important food legume crop that has large and complex genome. During past years, considerable attention has been given on the use of next generation sequencing for enriching the genomic resources including identification of SSR and SNP markers, development of unigenes, transcripts, and identification of candidate genes for biotic and abiotic stresses, analysis of genetic diversity and identification of genes/ QTLs for agronomically important traits. However, in other crops including pulses, next generation sequencing has revolutionized the genomic research and helped in genomic assisted breeding rapidly and cost effectively. The present review discuss current status and future prospects of the use NGS based breeding in lentil.

摘要

小扁豆是一种重要的食用豆科作物,具有庞大而复杂的基因组。在过去的几年中,人们对利用下一代测序技术来丰富基因组资源给予了相当多的关注,包括鉴定 SSR 和 SNP 标记、开发基因、转录本,并鉴定生物和非生物胁迫的候选基因、遗传多样性分析以及鉴定与农艺性状相关的基因/QTL。然而,在包括豆类在内的其他作物中,下一代测序技术已经彻底改变了基因组研究,并帮助进行基因组辅助育种,快速且经济有效地进行。本文讨论了基于下一代测序的小扁豆育种的现状和未来前景。

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