Department of Advanced Sciences and Technologies, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran; Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Division of Neurocognitive Sciences, Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
J Chem Neuroanat. 2020 Dec;110:101870. doi: 10.1016/j.jchemneu.2020.101870. Epub 2020 Oct 7.
Spinal cord injury (SCI) as a crippling disability causes tissue degeneration via neuron loss and fiber disruption. Some researchers have tried to reverse or minimize these changes. Platelet-rich plasma (PRP) is a biological product derived from peripheral blood containing a variety of growth factors. PRP has been extensively used in regenerative medicine. On the other hand, via secreting neuroprotective growth factors, mesenchymal stem cells (MSCs) have shown a promising potential in repairing central nervous system deficits. This study investigated the therapeutic effect of the combined use of MSCs and PRP in a rat model of SCI. We used real time-PCR method for evaluation of Bcl-2, Bax and caspase 3 expressions, TUNEL test for apoptotic cell death assessment, and neurofilament NF200 immunohistochemistry for examination of axonal regeneration. The results showed that co-treatment with MSCs and PRP efficiently alleviated the evaluated categories. Significant differences were observed in expression of Bcl-2 and caspase3, but not Bax, apoptotic index and the number of NF200 positive axons (for all P ≤ 0.01) between co-treatment animals compared with those treated with only MSCs or PRP. In conclusion, this study showed that combination of MSCs and PRP synergistically promotes their therapeutic effects in the SCI.
脊髓损伤（SCI）是一种致残性残疾，通过神经元丧失和纤维破坏导致组织退化。一些研究人员试图逆转或最小化这些变化。富含血小板的血浆（PRP）是一种源自外周血的生物制品，含有多种生长因子。PRP 已广泛用于再生医学。另一方面，间充质干细胞（MSCs）通过分泌神经保护生长因子，在修复中枢神经系统缺陷方面显示出很大的潜力。本研究探讨了 MSC 和 PRP 联合应用于 SCI 大鼠模型的治疗效果。我们使用实时 PCR 方法评估 Bcl-2、Bax 和 caspase 3 的表达，TUNEL 试验评估细胞凋亡，神经丝 NF200 免疫组化评估轴突再生。结果表明，MSC 和 PRP 的联合治疗能有效缓解评估指标。与仅接受 MSC 或 PRP 治疗的动物相比，联合治疗动物的 Bcl-2 和 caspase3 表达、凋亡指数和 NF200 阳性轴突数量均有显著差异（所有 P ≤ 0.01）。综上所述，本研究表明 MSC 和 PRP 的联合治疗可协同促进 SCI 的治疗效果。
