Department of Dentistry, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.
Department of Endodontics, School of Dentistry, Federal University of Pará, Belém, Pará, Brazil.
J Endod. 2021 Jan;47(1):105-111. doi: 10.1016/j.joen.2020.09.017. Epub 2020 Oct 9.
The purpose of this study was to evaluate the cleanliness of root canal walls and dentinal tubules after attempting to remove the calcium hydroxide dressing with different irrigant solutions and the use of nonactivated irrigation or passive ultrasonic irrigation (PUI).
After root canal instrumentation, 80 single-rooted teeth were filled with calcium hydroxide mixed with propylene glycol and 0.1% rhodamine B dye and inserted into canals with a Lentulo spiral. The calcium hydroxide dressing was initially removed with 10 mL saline solution and reinstrumentation with the master apical file. Then, the samples were randomly assigned into 8 experimental groups (n = 10) according to the irrigant solution with or without PUI: 2.5% sodium hypochlorite, 17% ethylenediaminetetraacetic acid + 1.25% sodium lauryl ether sulfate (EDTA-T), 37% phosphoric acid, or 70% ethanol. A final flush with 5 mL saline solution was performed. The percentage of clean root canal walls and the depth of clean dentinal tubules were measured with images of confocal laser scanning microscopy. The groups were compared using the 2-way analysis of variance test with the Bonferroni post hoc test for depth analysis and the Kruskal-Wallis with Dunn post hoc test for the perimeter analysis.
Irrigation with 70% ethanol presented a significantly higher percentage of clean root canal walls and a higher depth of clean dentinal tubules when compared with irrigation with 2.5% sodium hypochlorite and 17% EDTA-T for both irrigation methods (P < .05). No differences were observed between nonactivated irrigation or PUI protocols (P > .05).
Seventy percent ethanol enhanced calcium hydroxide removal from the apical root third compared with 2.5% sodium hypochlorite or 17% EDTA-T.
本研究旨在评估不同冲洗液在不使用和使用非激活冲洗或被动超声冲洗（PUI）的情况下尝试去除氢氧化钙封药后根管壁和牙本质小管的清洁程度。
根管器械预备后，将含有丙二醇和 0.1%罗丹明 B 染料的氢氧化钙与 Lentulo 螺旋混合后填充 80 颗单根牙，并将其插入根管内。最初用 10mL 生理盐水去除氢氧化钙封药，并用主尖锉重新处理。然后，根据有无 PUI 将样本随机分为 8 个实验组（n=10）：2.5%次氯酸钠、17%乙二胺四乙酸+1.25%十二烷基硫酸钠（EDTA-T）、37%磷酸或 70%乙醇。最后用 5mL 生理盐水冲洗。使用共聚焦激光扫描显微镜的图像测量根管壁清洁百分比和牙本质小管清洁深度。使用双向方差分析检验和 Bonferroni 事后检验进行深度分析，使用 Kruskal-Wallis 检验和 Dunn 事后检验进行周长分析，比较各组间差异。
与 2.5%次氯酸钠和 17%EDTA-T 相比，70%乙醇冲洗时根管壁清洁百分比和牙本质小管清洁深度均显著更高，无论采用哪种冲洗方法（P<.05）。非激活冲洗或 PUI 方案之间无差异（P>.05）。
与 2.5%次氯酸钠或 17%EDTA-T 相比，70%乙醇更能去除根尖 3 区的氢氧化钙。
