大肠杆菌K-12中鞭毛基因的转录调控
Transcriptional control of flagellar genes in Escherichia coli K-12.
作者信息
Komeda Y
出版信息
J Bacteriol. 1986 Dec;168(3):1315-8. doi: 10.1128/jb.168.3.1315-1318.1986.
Abstract
Autoregulation of the expression of flagellar genes was investigated by the technique of operon fusion. The results suggested that the flaU gene is a repressor and the flaD gene is an activator of transcription of the hag, flaS, and Mocha operons. The action of the putative flaU repressor appears to be masked by its interaction with other flagellar proteins during assembly; thus, repression is apparent only when the interacting proteins are absent. This hypothesis is supported by the phenotype of an unusual flaU mutant, which represses even though it is unable to promote flagellar assembly. Presumably, the mutant synthesizes a repressor whose activity is no longer masked by interaction with other flagellar proteins.
摘要
通过操纵子融合技术研究了鞭毛基因表达的自动调节。结果表明，flaU基因是一种阻遏物，而flaD基因是hag、flaS和Mocha操纵子转录的激活物。假定的flaU阻遏物的作用在组装过程中似乎被其与其他鞭毛蛋白的相互作用所掩盖；因此，只有在相互作用的蛋白不存在时，阻遏作用才明显。这一假设得到了一个异常flaU突变体表型的支持，该突变体即使无法促进鞭毛组装也能产生阻遏作用。据推测，该突变体合成了一种阻遏物，其活性不再被与其他鞭毛蛋白的相互作用所掩盖。
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本文引用的文献
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