大肠杆菌K-12中鞭毛基因座的缺失和琥珀突变体
Deletion and amber mutants of fla loci in Escherichia coli K-12.
作者信息
Kondoh H, Ozeki H
出版信息
Genetics. 1976 Nov;84(3):403-21. doi: 10.1093/genetics/84.3.403.
Abstract
A rapid screening method for amber fla mutants of E. coli was devised and many mutants were obtained. In addition, strains with deletions of the fla genes in the his-uvrC region were isolated from high-temperature survivors of a lambda cI857 lysogen in which the prophage is located between his and fla. Utilizing these mutants, eleven fla genes (I--XI) and one hag gene were identified in the his-uvrc region, in the following order: his-supD-I-II-(III, IV)-V-(VI, VII)-VIII-IX-hag-(X, XI)-uvrC. The fla genes X and XI and hag are located at about 42.5 min and the other fla genes at about 43.0 min on the E. coli genetic map (Bachmann, Low and Taylor 1976). Mutants of fla gene X showed a slight sensitivity to chi phage, although they lack the flagellar filament.
摘要
设计了一种用于快速筛选大肠杆菌琥珀色fla突变体的方法，并获得了许多突变体。此外，从λcI857溶原菌的高温存活菌株中分离出了his-uvrC区域fla基因缺失的菌株，其中原噬菌体位于his和fla之间。利用这些突变体，在his-uvrc区域按以下顺序鉴定出11个fla基因（I--XI）和1个hag基因：his-supD-I-II-(III, IV)-V-(VI, VII)-VIII-IX-hag-(X, XI)-uvrC。在大肠杆菌遗传图谱上（Bachmann、Low和Taylor，1976），fla基因X和XI以及hag位于约42.5分钟处，其他fla基因位于约43.0分钟处。fla基因X的突变体虽然缺乏鞭毛丝，但对chi噬菌体表现出轻微的敏感性。
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