Laboratory of Biochemistry and Biomedical Materials, College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, PR China; Laboratory for Marine Drugs and Bioproducts of Pilot National Laboratory for Marine Science and Technology, Qingdao, 266235, PR China.
Laboratory of Biochemistry and Biomedical Materials, College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, PR China.
Carbohydr Polym. 2020 Dec 15;250:116994. doi: 10.1016/j.carbpol.2020.116994. Epub 2020 Aug 28.
Herein, the effects of carboxymethyl chitosan oligosaccharide (CM-COS) on regulating immunologic function and inhibiting hepatocellular tumor growth were evaluated. Results showed that CM-COS caused dramatic viability loss of hepatocellular carcinoma BEL-7402 with non-toxicity towards normal liver L-02 cells. CM-COS repressed tumor growth of hepatoma-22, and elevated the spleen index and thymus index of tumor-bearing mice. Contents of VEGF and MMP-9 were significantly down-regulated by CM-COS. Histological analyses revealed that CM-COS promoted tumor cell necrosis and produced no significant toxicity to spleen tissues. Moreover, expressions of Caspase-3 in tumor tissues and IL-2 in spleen tissues were significantly activated by CM-COS. Additionally, in vitro cell viability, phagocytic capability and NO production of mouse peritoneal macrophages exposed to CM-COS were significantly higher. CM-COS remarkably increased the in vivo phagocytosing capacity of peritoneal macrophages of Kunming mice. Taken together, our findings suggested that CM-COS might be potentially effective and non-toxic candidate as anti-hepatoma agents.
本文评价了羧甲基壳聚糖寡糖（CM-COS）对调节免疫功能和抑制肝癌生长的影响。结果表明，CM-COS 对正常肝细胞 L-02 无毒性，但对肝癌 BEL-7402 细胞有明显的杀伤作用。CM-COS 抑制肝癌-22 的生长，并提高荷瘤小鼠的脾脏指数和胸腺指数。CM-COS 显著下调 VEGF 和 MMP-9 的含量。组织学分析表明，CM-COS 促进肿瘤细胞坏死，对脾组织无明显毒性。此外，CM-COS 显著激活肿瘤组织中的 Caspase-3 和脾组织中的 IL-2 的表达。此外，CM-COS 显著提高了体外培养的小鼠腹腔巨噬细胞的细胞活力、吞噬能力和 NO 产生。CM-COS 显著增加了昆明小鼠腹腔巨噬细胞的体内吞噬能力。综上所述，CM-COS 可能是一种有潜力的、无毒的抗肝癌候选药物。
