School of Pharmacy, Shandong University of Traditional Chinese Medicine (TCM), Jinan, 250355, P. R. China.
Shandong Drug and Food Vocational College, Weihai, 264210, P. R. China.
Chem Biodivers. 2020 Dec;17(12):e2000666. doi: 10.1002/cbdv.202000666. Epub 2020 Nov 6.
The continued development of folk medicine to potentially treat infectious diseases has resulted in an increase in natural sources of antimicrobial agents, particularly the use of plant essential oils containing volatile products from secondary metabolism. The objectives of this investigation were to (i) analyze the chemical components of essential oils using GC/MS and (ii) to examine their in vitro antimicrobial activities against four strains of bacteria (Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Shigella flexneri) and one fungus (Candida albicans) by determining minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) in liquid and solid media, respectively, from different Pyrrosia petiolosa locations. Eighty-eight evaporable compounds were confirmed in their essential oils; the major components in the oils were 2,4-pentadienal (12.5 %), phytol (10.5 %) and nonanal (8.6 %). Based on hierarchical cluster analysis, Pyrrosia samples were categorized into four groups, indicating significant essential oil diversity from different Pyrrosia locations. Results also indicated that essential oils had a broad spectrum of antibacterial activities, particularly against Shigella flexneri and Staphylococcus aureus with MICs of 5 μL/mL. Results from this investigation are the first to record the chemical component and antimicrobial potential of essential oils from different P. Petiolosa locations.
民间医学的持续发展有可能治疗传染病，这导致了更多天然来源的抗菌剂的出现，特别是使用含有次级代谢挥发产物的植物精油。本研究的目的是：（i）使用 GC/MS 分析精油的化学成分；（ii）通过在液体和固体培养基中分别确定最小抑菌浓度（MICs）和最小杀菌浓度（MBCs），研究来自不同荷叶离褶伞产地的精油对 4 种细菌（金黄色葡萄球菌、枯草芽孢杆菌、大肠杆菌和福氏志贺菌）和 1 种真菌（白色念珠菌）的体外抗菌活性。从其精油中鉴定出 88 种可挥发化合物；油中的主要成分是 2,4-戊二烯醛（12.5％）、叶绿醇（10.5％）和壬醛（8.6％）。基于层次聚类分析，荷叶离褶伞样品分为 4 组，表明不同荷叶离褶伞产地的精油具有显著的多样性。结果还表明，精油具有广谱的抗菌活性，特别是对福氏志贺菌和金黄色葡萄球菌的 MIC 为 5μL/mL。本研究结果首次记录了不同荷叶离褶伞产地精油的化学成分和抗菌潜力。
