Laboratory of Natural Substances, National Institute of Research and Physico-Chemical Analyses, Biotechpole of Sidi Thabet, Ariana, 2020, Tunisia (phone: +216-71537666; fax: +216-71537688).
Chem Biodivers. 2013 Dec;10(12):2278-90. doi: 10.1002/cbdv.201300137.
The essential oils of Daucus carota L. (Apiaceae) seeds sampled from ten wild populations spread over northern Tunisia were characterized by GC-FID and GC/MS analyses. In total, 36 compounds were identified in the D. carota seed essential oils, with a predominance of sesquiterpene hydrocarbons in most samples (22.63-89.93% of the total oil composition). The main volatile compounds identified were β-bisabolene (mean content of 39.33%), sabinene (8.53%), geranyl acetate (7.12%), and elemicin (6.26%). The volatile composition varied significantly across the populations, even for oils of populations harvested in similar areas. The chemometric principal component analysis and the hierarchical clustering identified four groups, each corresponding to a composition-specific chemotype. The in vitro antimicrobial activity of the isolated essential oils was preliminarily evaluated, using the disk-diffusion method, against one Gram-positive (Staphylococcus aureus) and two Gram-negative bacteria (Escherichia coli and Salmonella typhimurium), as well as against a pathogenic yeast (Candida albicans). All tested essential oils exhibited interesting antibacterial and antifungal activities against the assayed microorganisms.
从分布于突尼斯北部的十个野生种群中采集的胡萝卜（伞形科）种子精油通过 GC-FID 和 GC/MS 分析进行了表征。在 D. carota 种子精油中总共鉴定出 36 种化合物，大多数样品中以倍半萜烃为主（占总油组成的 22.63％-89.93％）。鉴定出的主要挥发性化合物为β-倍半萜烯（平均含量为 39.33％），柠檬烯（8.53％），乙酸香叶酯（7.12％）和 elemicin（6.26％）。挥发性成分在种群间存在显著差异，即使在相似地区收获的种群的油也是如此。化学计量主成分分析和层次聚类确定了四个组，每个组对应于特定组成的化学型。使用圆盘扩散法初步评估了分离的精油对一种革兰氏阳性（金黄色葡萄球菌）和两种革兰氏阴性菌（大肠杆菌和鼠伤寒沙门氏菌）以及一种致病性酵母（白色念珠菌）的体外抗菌活性。所有测试的精油对测试的微生物均表现出有趣的抗菌和抗真菌活性。
