Wadström T, Andersson K, Sydow M, Axelsson L, Lindgren S, Gullmar B
J Appl Bacteriol. 1987 Jun;62(6):513-20. doi: 10.1111/j.1365-2672.1987.tb02683.x.
One hundred wild-type strains of the genus Lactobacillus were isolated from the small intestine of newly-slaughtered pigs up to 6 months of age. Cell surface hydrophobicity and capsule formation were studied on a number of strains. Strains showing high surface hydrophobicity as measured by the salt-aggregation test and hydrophobic interaction chromatography on Octyl Sepharose were commonly found to adhere in high numbers to isolated pig intestinal epithelial cells. Heat and protease treatment of bacteria of high surface hydrophobicity, including autoaggregating strains in phosphate-buffered saline, showed a drastic decline in this surface property. Three hydrophilic strains (LBp 1044, 1068 and 1073) also showed binding to intestinal cells but at a lower level (approx. 5 bacteria/cell) as compared with the best binding hydrophobic strain (LBp 1063, approx. 11 bacteria/cell). These findings suggest that different or multiple adhesion mechanisms may be involved in the colonization of the small intestinal mucosa of pigs. Cultures of selected strains grown in liquid media rich in carbohydrates did not affect their hydrophobic cell surface character. Therefore it seems less likely that carbohydrate capsule polymers are the major determinants of intestinal colonization of lactobacilli in pigs.
从6月龄以内刚屠宰的猪的小肠中分离出100株野生型乳酸杆菌菌株。对许多菌株进行了细胞表面疏水性和荚膜形成的研究。通过盐聚集试验和辛基琼脂糖凝胶上的疏水相互作用色谱法测定，显示出高表面疏水性的菌株通常被发现大量粘附于分离出的猪肠上皮细胞。对包括在磷酸盐缓冲盐水中自动聚集的菌株在内的高表面疏水性细菌进行加热和蛋白酶处理后，这种表面特性急剧下降。三株亲水性菌株（LBp 1044、1068和1073）也显示出与肠细胞的结合，但与结合能力最强的疏水性菌株（LBp 1063，约11个细菌/细胞）相比，结合水平较低（约5个细菌/细胞）。这些发现表明，猪小肠黏膜定植可能涉及不同或多种粘附机制。在富含碳水化合物的液体培养基中培养的选定菌株的培养物不会影响其疏水性细胞表面特征。因此，碳水化合物荚膜聚合物似乎不太可能是猪中乳酸杆菌肠道定植的主要决定因素。
