1Department of Clinical Diagnostics, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-957, Olsztyn, Poland.
2Department of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
Acta Vet Hung. 2020 Oct 13;68(2):154-159. doi: 10.1556/004.2020.00028.
Due to its difficult diagnosis and complicated treatment, inflammatory bowel disease (IBD) in dogs is a challenge for the veterinarian. Several aspects connected with pathological changes during IBD still remain unknown. Since one of these aspects is the participation of intestinal innervation in the evolution of the disease, the aim of this study was to demonstrate changes in the number and distribution of intramucosal colonic nerve fibres immunoreactive to substance P (SP) arising as the disease progresses. SP is one of the most important neuronal factors in intestinal innervation which, among other tasks, takes part in the conduction of pain stimuli. Using routine immunofluorescence technique, the density of nerve fibres containing SP was evaluated within mucosal biopsy specimens collected from the descending colon of healthy dogs and animals suffering from IBD of varying severity. The results of the study indicate that during severe IBD the number of nerve fibres containing SP located in the colonic mucosal layer increases in comparison to control animals. The number of SP-positive intramucosal nerves amounted to 10.99 ± 2.11 nerves per observation field in healthy dogs, 14.62 ± 2.86 in dogs with mild IBD, 14.80 ± 0.91 in dogs with moderate IBD and 19.03 ± 6.11 in animals with severe IBD. The observed changes were directly proportional to the intensity of the disease process. These observations may suggest a role of this neuronal substance in pathological processes occurring during IBD. Although the exact mechanism of the observed changes has not been completely explained, the results obtained in this investigation may contribute to improving the diagnosis and treatment of this disease, as well as the staging of canine IBD in veterinary practice.
由于其诊断困难和治疗复杂，犬的炎症性肠病 (IBD) 对兽医来说是一个挑战。IBD 过程中的一些病理变化仍然未知。由于这些方面之一是肠神经支配参与疾病的演变，因此本研究的目的是证明随着疾病的进展，对 P 物质 (SP) 呈免疫反应的粘膜内结肠神经纤维的数量和分布发生变化。SP 是肠神经支配中最重要的神经元因子之一，除其他任务外，它还参与疼痛刺激的传导。使用常规免疫荧光技术，评估来自健康犬和患有不同严重程度 IBD 的动物的降结肠粘膜活检标本中含有 SP 的神经纤维的密度。研究结果表明，在严重的 IBD 期间，与对照动物相比，位于结肠粘膜层中的含有 SP 的神经纤维数量增加。在健康犬中，SP 阳性粘膜内神经的数量为每观察视野 10.99 ± 2.11 条神经，轻度 IBD 犬为 14.62 ± 2.86 条神经，中度 IBD 犬为 14.80 ± 0.91 条神经，严重 IBD 动物为 19.03 ± 6.11 条神经。观察到的变化与疾病过程的强度成正比。这些观察结果可能表明这种神经元物质在 IBD 发生的病理过程中起作用。尽管尚未完全解释观察到的变化的确切机制，但本研究中的结果可能有助于改善该疾病的诊断和治疗，以及兽医实践中犬 IBD 的分期。
