用无色肽酶提取法对β溶血性链球菌单菌落进行血清分型。
Serogrouping single colonies of beta-hemolytic streptococci with achromopeptidase extraction.
作者信息
Slifkin M, Cumbie R
出版信息
J Clin Microbiol. 1987 Aug;25(8):1555-6. doi: 10.1128/jcm.25.8.1555-1556.1987.
Abstract
Achromopeptidase (TBL-1; Wako Chemicals, USA, Inc., Dallas, Tex.) prepared as a 2,000-U/ml solution will extract the serogroup antigens from single colonies of groups A, B, C, F, and G streptococci in 1 min at room temperature. This enzyme extraction is not effective for the serogrouping of all group D streptococcus species. Achromopeptidase extracts can be used with latex or coagglutination reagents.
摘要
无色肽酶（TBL-1；和光纯药工业株式会社美国公司，得克萨斯州达拉斯）配制成2000 U/ml的溶液，在室温下1分钟内可从A、B、C、F和G群链球菌的单个菌落中提取血清群抗原。这种酶提取法对所有D群链球菌种的血清学分型无效。无色肽酶提取物可与乳胶或协同凝集试剂一起使用。
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