• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

在菌血症期间，铜绿假单胞菌 PAO1 通过改变许多毒力基因的表达来适应，包括那些参与群体感应的基因。

During bacteremia, Pseudomonas aeruginosa PAO1 adapts by altering the expression of numerous virulence genes including those involved in quorum sensing.

机构信息

Department of Immunology and Molecular Microbiology, Texas Tech University Health Sciences Center, Lubbock, Texas, Untied States of America.

Honors College, Texas Tech University, Lubbock, Texas, Untied States of America.

出版信息

PLoS One. 2020 Oct 15;15(10):e0240351. doi: 10.1371/journal.pone.0240351. eCollection 2020.

DOI:10.1371/journal.pone.0240351
PMID:33057423
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7561203/
Abstract

Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen that produces numerous virulence factors and causes serious infections in trauma patients and patients with severe burns. We previously showed that the growth of P. aeruginosa in blood from severely burned or trauma patients altered the expression of numerous genes. However, the specific influence of whole blood from healthy volunteers on P. aeruginosa gene expression is not known. Transcriptome analysis of P. aeruginosa grown for 4 h in blood from healthy volunteers compared to that when grown in laboratory medium revealed that the expression of 1085 genes was significantly altered. Quorum sensing (QS), QS-related, and pyochelin synthesis genes were downregulated, while genes of the type III secretion system and those for pyoverdine synthesis were upregulated. The observed effect on the QS and QS-related genes was shown to reside within serum fraction: growth of PAO1 in the presence of 10% human serum from healthy volunteers significantly reduced the expression of QS and QS-regulated genes at 2 and 4 h of growth but significantly enhanced their expression at 8 h. Additionally, the production of QS-regulated virulence factors, including LasA and pyocyanin, was also influenced by the presence of human serum. Serum fractionation experiments revealed that part of the observed effect resides within the serum fraction containing <10-kDa proteins. Growth in serum reduced the production of many PAO1 outer membrane proteins but enhanced the production of others including OprF, a protein previously shown to play a role in the regulation of QS gene expression. These results suggest that factor(s) within human serum: 1) impact P. aeruginosa pathogenesis by influencing the expression of different genes; 2) differentially regulate the expression of QS and QS-related genes in a growth phase- or time-dependent mechanism; and 3) manipulate the production of P. aeruginosa outer membrane proteins.

摘要

铜绿假单胞菌是一种革兰氏阴性机会性病原体，它产生许多毒力因子，并导致创伤患者和严重烧伤患者发生严重感染。我们之前的研究表明，来自严重烧伤或创伤患者的血液中铜绿假单胞菌的生长改变了许多基因的表达。然而，健康志愿者的全血对铜绿假单胞菌基因表达的具体影响尚不清楚。与在实验室培养基中生长相比，将铜绿假单胞菌在健康志愿者血液中生长 4 小时的转录组分析显示，有 1085 个基因的表达显著改变。群体感应（QS）、QS 相关和焦脱镁叶绿酸合成基因下调，而 III 型分泌系统基因和吡咯并嘧啶合成基因上调。观察到对 QS 和 QS 相关基因的影响存在于血清部分：在存在来自健康志愿者的 10%人血清的情况下，PAO1 的生长在 2 和 4 小时的生长显著降低了 QS 和 QS 调节基因的表达，但在 8 小时的生长显著增强了它们的表达。此外，QS 调节的毒力因子的产生，包括 LasA 和绿脓菌素，也受到人血清的影响。血清部分分离实验表明，观察到的部分效应存在于含有<10 kDa 蛋白的血清部分中。在血清中生长会减少许多 PAO1 外膜蛋白的产生，但会增强其他蛋白的产生，包括 OprF，OprF 先前被证明在调节 QS 基因表达中起作用。这些结果表明，人血清中的因素：1）通过影响不同基因的表达来影响铜绿假单胞菌的发病机制；2）以生长阶段或时间依赖的机制差异调节 QS 和 QS 相关基因的表达；3）操纵铜绿假单胞菌外膜蛋白的产生。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/7561203/5aa56fea99b5/pone.0240351.g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/7561203/2426943a934d/pone.0240351.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/7561203/1ddf86d69237/pone.0240351.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/7561203/0c14d1fc46ab/pone.0240351.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/7561203/c787ae9c2b2b/pone.0240351.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/7561203/a86d195477bb/pone.0240351.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/7561203/b01f8de4e393/pone.0240351.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/7561203/cf5164d5168b/pone.0240351.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/7561203/302f824c76bb/pone.0240351.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/7561203/008f1b097afc/pone.0240351.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/7561203/67eaa0c095f6/pone.0240351.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/7561203/77e45a526210/pone.0240351.g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/7561203/07f395617883/pone.0240351.g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/7561203/5aa56fea99b5/pone.0240351.g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/7561203/2426943a934d/pone.0240351.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/7561203/1ddf86d69237/pone.0240351.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/7561203/0c14d1fc46ab/pone.0240351.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/7561203/c787ae9c2b2b/pone.0240351.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/7561203/a86d195477bb/pone.0240351.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/7561203/b01f8de4e393/pone.0240351.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/7561203/cf5164d5168b/pone.0240351.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/7561203/302f824c76bb/pone.0240351.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/7561203/008f1b097afc/pone.0240351.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/7561203/67eaa0c095f6/pone.0240351.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/7561203/77e45a526210/pone.0240351.g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/7561203/07f395617883/pone.0240351.g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/7561203/5aa56fea99b5/pone.0240351.g013.jpg

相似文献

1
During bacteremia, Pseudomonas aeruginosa PAO1 adapts by altering the expression of numerous virulence genes including those involved in quorum sensing.在菌血症期间，铜绿假单胞菌 PAO1 通过改变许多毒力基因的表达来适应，包括那些参与群体感应的基因。
PLoS One. 2020 Oct 15;15(10):e0240351. doi: 10.1371/journal.pone.0240351. eCollection 2020.
2
Differential Modulation of Quorum Sensing Signaling through QslA in Pseudomonas aeruginosa Strains PAO1 and PA14.通过铜绿假单胞菌 PAO1 和 PA14 中的 QslA 对群体感应信号进行差异调节。
J Bacteriol. 2019 Oct 4;201(21). doi: 10.1128/JB.00362-19. Print 2019 Nov 1.
3
Serum influences the expression of Pseudomonas aeruginosa quorum-sensing genes and QS-controlled virulence genes during early and late stages of growth.在生长的早期和晚期，血清会影响铜绿假单胞菌群体感应基因以及群体感应控制的毒力基因的表达。
Microbiologyopen. 2014 Feb;3(1):64-79. doi: 10.1002/mbo3.147.
4
The Small RNA AmiL Regulates Quorum Sensing-Mediated Virulence in Pseudomonas aeruginosa PAO1.小 RNA AmiL 调控铜绿假单胞菌 PAO1 群体感应介导的毒力。
Microbiol Spectr. 2022 Apr 27;10(2):e0221121. doi: 10.1128/spectrum.02211-21. Epub 2022 Mar 9.
5
Major Transcriptome Changes Accompany the Growth of Pseudomonas aeruginosa in Blood from Patients with Severe Thermal Injuries.严重热损伤患者血液中铜绿假单胞菌生长伴随主要转录组变化。
PLoS One. 2016 Mar 2;11(3):e0149229. doi: 10.1371/journal.pone.0149229. eCollection 2016.
6
Diallyl disulfide from garlic oil inhibits Pseudomonas aeruginosa virulence factors by inactivating key quorum sensing genes.大蒜油中的二烯丙基二硫通过使关键群体感应基因失活来抑制铜绿假单胞菌毒力因子。
Appl Microbiol Biotechnol. 2018 Sep;102(17):7555-7564. doi: 10.1007/s00253-018-9175-2. Epub 2018 Jun 27.
7
Sodium salicylate interferes with quorum-sensing-regulated virulence in chronic wound isolates of in simulated wound fluid.水杨酸钠会干扰慢性伤口分离株中群体感应调节的毒力在模拟伤口液中。
J Med Microbiol. 2020 May;69(5):767-780. doi: 10.1099/jmm.0.001188. Epub 2020 Apr 22.
8
Ellagic acid derivatives from Terminalia chebula Retz. downregulate the expression of quorum sensing genes to attenuate Pseudomonas aeruginosa PAO1 virulence.没食子酸衍生物对 Terminalia chebula Retz. 的下调表达可调节群体感应基因的表达，从而减轻铜绿假单胞菌 PAO1 的毒力。
PLoS One. 2013;8(1):e53441. doi: 10.1371/journal.pone.0053441. Epub 2013 Jan 8.
9
Affecting Pseudomonas aeruginosa phenotypic plasticity by quorum sensing dysregulation hampers pathogenicity in murine chronic lung infection.通过群体感应失调影响铜绿假单胞菌的表型可塑性会阻碍小鼠慢性肺部感染中的致病性。
PLoS One. 2014 Nov 24;9(11):e112105. doi: 10.1371/journal.pone.0112105. eCollection 2014.
10
RpoN-Dependent Direct Regulation of Quorum Sensing and the Type VI Secretion System in Pseudomonas aeruginosa PAO1.RpoN 依赖性群体感应和铜绿假单胞菌 PAO1 中 VI 型分泌系统的直接调控。
J Bacteriol. 2018 Jul 25;200(16). doi: 10.1128/JB.00205-18. Print 2018 Aug 15.

引用本文的文献

1
Transcriptomic and functional profiling of reveals adaptation to burn patient blood and time-dependent responses to human serum.对……的转录组学和功能分析揭示了对烧伤患者血液的适应性以及对人血清的时间依赖性反应。 你提供的原文中“of”后面缺少具体内容，请补充完整以便能准确翻译。
Front Microbiol. 2025 Aug 29;16:1635690. doi: 10.3389/fmicb.2025.1635690. eCollection 2025.
2
Exploring the antibacterial potential of environmental Pseudomonas aeruginosa isolates: Insights from in vitro studies and genome mining approaches.探索环境中分离出的铜绿假单胞菌的抗菌潜力：来自体外研究和基因组挖掘方法的见解。
J Genet Eng Biotechnol. 2025 Sep;23(3):100508. doi: 10.1016/j.jgeb.2025.100508. Epub 2025 May 17.
3

本文引用的文献

1
Mechanism of pyocyanin abolishment caused by double knockout in PAO1.PAO1 双基因敲除导致绿脓菌素消除的机制。
Virulence. 2019 Dec;11(1):57-67. doi: 10.1080/21505594.2019.1708052.
2
ShinyGO: a graphical gene-set enrichment tool for animals and plants.ShinyGO：一个用于动植物的图形基因集富集工具。
Bioinformatics. 2020 Apr 15;36(8):2628-2629. doi: 10.1093/bioinformatics/btz931.
3
The clinical value of IL-3, IL-4, IL-12p70, IL17A, IFN-γ, MIP-1β, NLR, P-selectin, and TNF-α in differentiating bloodstream infections caused by gram-negative, gram-positive bacteria and fungi in hospitalized patients: An Observational Study.
Human whole blood influences the expression of Acinetobacter baumannii genes related to translation and siderophore production.
人全血会影响鲍曼不动杆菌中与翻译和铁载体产生相关的基因表达。
PLoS One. 2025 Jul 24;20(7):e0326330. doi: 10.1371/journal.pone.0326330. eCollection 2025.
4
A purified and lyophilized Pseudomonas aeruginosa derived pyocyanin induces promising apoptotic and necrotic activities against MCF-7 human breast adenocarcinoma.一株经纯化和冻干的铜绿假单胞菌衍生绿脓菌素对 MCF-7 人乳腺癌腺癌细胞具有显著的促凋亡和促坏死活性。
Microb Cell Fact. 2022 Dec 17;21(1):262. doi: 10.1186/s12934-022-01988-x.
5
reference strains PAO1 and PA14: A genomic, phenotypic, and therapeutic review.参考菌株PAO1和PA14：基因组、表型及治疗学综述
Front Microbiol. 2022 Oct 13;13:1023523. doi: 10.3389/fmicb.2022.1023523. eCollection 2022.
6
Decoding Genetic Features and Antimicrobial Susceptibility of Strains Isolated from Bloodstream Infections.解析血流感染分离株的遗传特征和抗菌药敏性
Int J Mol Sci. 2022 Aug 16;23(16):9208. doi: 10.3390/ijms23169208.
7
Novel Insight of Transcription Factor PtrA on Pathogenicity and Carbapenems Resistance in .转录因子PtrA对……致病性和碳青霉烯类耐药性的新见解
Infect Drug Resist. 2022 Aug 4;15:4213-4227. doi: 10.2147/IDR.S371597. eCollection 2022.
8
Pseudomonas aeruginosa clinical blood isolates display significant phenotypic variability.铜绿假单胞菌临床血分离株表现出显著的表型变异性。
PLoS One. 2022 Jul 6;17(7):e0270576. doi: 10.1371/journal.pone.0270576. eCollection 2022.
9
High pyocyanin production and non-motility of Pseudomonas aeruginosa isolates are correlated with septic shock or death in bacteremic patients.铜绿假单胞菌分离株中高吡咯菌素的产生和非运动性与菌血症患者的感染性休克或死亡相关。
PLoS One. 2021 Jun 11;16(6):e0253259. doi: 10.1371/journal.pone.0253259. eCollection 2021.
10
Malonate utilization by Pseudomonas aeruginosa affects quorum-sensing and virulence and leads to formation of mineralized biofilm-like structures.铜绿假单胞菌利用丙二酸盐会影响群体感应和毒力，并导致形成矿化的生物膜样结构。
Mol Microbiol. 2021 Aug;116(2):516-537. doi: 10.1111/mmi.14729. Epub 2021 May 18.
IL-3、IL-4、IL-12p70、IL17A、IFN-γ、MIP-1β、中性粒细胞与淋巴细胞比值（NLR）、P-选择素及肿瘤坏死因子-α（TNF-α）在区分住院患者革兰阴性菌、革兰阳性菌及真菌所致血流感染中的临床价值：一项观察性研究
Medicine (Baltimore). 2019 Sep;98(38):e17315. doi: 10.1097/MD.0000000000017315.
4
Pseudomonas aeruginosa Alters Its Transcriptome Related to Carbon Metabolism and Virulence as a Possible Survival Strategy in Blood from Trauma Patients.铜绿假单胞菌改变其与碳代谢和毒力相关的转录组，作为在创伤患者血液中可能的生存策略。
mSystems. 2019 May 7;4(4). doi: 10.1128/mSystems.00312-18. eCollection 2019 Jul-Aug.
5
Fitting Pieces into the Puzzle of Type III Secretion System Gene Expression.将碎片拼入 III 型分泌系统基因表达的谜题中。
J Bacteriol. 2019 Jun 10;201(13). doi: 10.1128/JB.00209-19. Print 2019 Jul 1.
6
Evolution of the quorum-sensing hierarchy.群体感应调控层级的进化。
Proc Natl Acad Sci U S A. 2019 Apr 2;116(14):7027-7032. doi: 10.1073/pnas.1819796116. Epub 2019 Mar 8.
7
The Gene Ontology Resource: 20 years and still GOing strong.《基因本体论资源：20 年，持续强大》
Nucleic Acids Res. 2019 Jan 8;47(D1):D330-D338. doi: 10.1093/nar/gky1055.
8
Rhamnolipids from disperse the biofilms of sulfate-reducing bacteria.来自[具体来源未提及]的鼠李糖脂可分散硫酸盐还原菌的生物膜。
NPJ Biofilms Microbiomes. 2018 Oct 3;4:22. doi: 10.1038/s41522-018-0066-1. eCollection 2018.
9
Blood or Serum Exposure Induce Global Transcriptional Changes, Altered Antigenic Profile, and Increased Cytotoxicity by Classical Bordetellae.血液或血清暴露会引发全局转录变化、抗原谱改变，并增加经典博德特氏菌的细胞毒性。
Front Microbiol. 2018 Sep 7;9:1969. doi: 10.3389/fmicb.2018.01969. eCollection 2018.
10
Differential Regulation of the Phenazine Biosynthetic Operons by Quorum Sensing in PAO1-N.群体感应调控 PAO1-N 中吩嗪生物合成操纵子的差异表达
Front Cell Infect Microbiol. 2018 Jul 23;8:252. doi: 10.3389/fcimb.2018.00252. eCollection 2018.