Gestal Monica C, Rivera Israel, Howard Laura K, Dewan Kalyan K, Soumana Illiassou Hamidou, Dedloff Margaret, Nicholson Tracy L, Linz Bodo, Harvill Eric T
Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA, United States.
USDA ARS National Animal Disease Center, Ames, IA, United States.
Front Microbiol. 2018 Sep 7;9:1969. doi: 10.3389/fmicb.2018.01969. eCollection 2018.
The classical bordetellae sense and respond to a variety of environments outside and within their mammalian hosts. By causing inflammation and tissue damage, we reasoned that bordetellae are likely to encounter components of blood and/or serum during the course of a respiratory infection, and that detecting and responding to these would be advantageous. Therefore, we hypothesized that classical bordetellae have the ability to sense and respond to blood or serum. Blood or serum exposure resulted in substantial transcriptional changes in , including enhanced expression of many virulence-associated genes. Exposure to blood or serum additionally elicited production of multiple antigens not otherwise detectable, and led to increased bacterial cytotoxicity against macrophages. Transcriptional responses to blood/serum were observed in a Bvg phase-locked mutant, indicating that the response is not solely dependent on a functional BvgAS system. Similar transcriptional responses to blood/serum were observed for the other classical bordetellae, and These data suggest the classical bordetellae respond to signals present in blood and serum by changing their behavior in ways that likely contribute to their remarkable success, via effects on pathogenesis, persistence and/or transmission between hosts.
经典博德特氏菌能够感知并响应其哺乳动物宿主内外的多种环境。我们推断，由于引发炎症和组织损伤，博德特氏菌在呼吸道感染过程中可能会接触到血液和/或血清成分，而检测并响应这些成分会具有优势。因此，我们假设经典博德特氏菌具有感知并响应血液或血清的能力。血液或血清暴露导致了显著的转录变化，包括许多毒力相关基因的表达增强。暴露于血液或血清还引发了多种其他情况下无法检测到的抗原的产生，并导致细菌对巨噬细胞的细胞毒性增加。在一个Bvg相位锁定突变体中观察到了对血液/血清的转录反应，这表明该反应并非仅依赖于功能性的BvgAS系统。对于其他经典博德特氏菌也观察到了类似的对血液/血清的转录反应。这些数据表明，经典博德特氏菌通过改变其行为来响应血液和血清中存在的信号，这些改变可能通过对发病机制、持续性和/或宿主间传播的影响，对它们的显著成功起到了促进作用。
