Polimerbio SL, Donostia-San Sebastian, Spain.
Department of Cell Biology and Histology, Faculty of Medicine and Nursing, University of the Basque Country (UPV/EHU), Leioa, Spain.
Nanomedicine. 2021 Jan;31:102314. doi: 10.1016/j.nano.2020.102314. Epub 2020 Oct 12.
Within the field of neural tissue engineering, there is a huge need for the development of materials that promote the adhesion, aligned migration and differentiation of stem cells into neuronal and supportive glial cells. In this study, we have fabricated bioresorbable elastomeric scaffolds combining an ordered nanopatterned topography together with a surface functionalization with graphene oxide (GO) in mild conditions. These scaffolds allowed the attachment of murine neural stem cells (NSCs) without the need of any further coating of its surface with extracellular matrix adhesion proteins. The NSCs were able to give rise to both immature neurons and supporting glial cells over the nanostructured scaffolds in vitro, promoting their aligned migration in cell clusters following the nanostructured grooves. This system has the potential to reestablish spatially oriented neural precursor cell connectivity, constituting a promising tool for future cellular therapy including nerve tissue regeneration.
在神经组织工程领域，人们急需开发出能促进干细胞黏附、定向迁移并向神经元和支持性神经胶质细胞分化的材料。在本研究中，我们通过温和的条件制备了一种生物可吸收弹性体支架，该支架将有序的纳米图案形貌与氧化石墨烯（GO）的表面功能化结合在一起。这些支架允许鼠神经干细胞（NSCs）黏附，而无需进一步用细胞外基质黏附蛋白对其表面进行涂层。在体外，NSCs 能够在纳米结构支架上产生未成熟神经元和支持性神经胶质细胞，并在纳米结构沟槽的引导下促进细胞簇的定向迁移。该系统具有重建空间定向神经前体细胞连接的潜力，为未来的细胞治疗，包括神经组织再生，提供了一种很有前途的工具。
