Yulizal O K, Lelo Aznan, Ilyas Syafruddin, Kusumawati Raden Lia
Department of Internal Medicine, Faculty of Medicine, Universitas Prima Indonesia, Medan, Indonesia.
School of Medicine, Universitas Sumatera Utara, Medan, Indonesia.
Vet World. 2020 Aug;13(8):1605-1612. doi: 10.14202/vetworld.2020.1605-1612. Epub 2020 Aug 17.
The presence of gastric mucosa or submucosa inflammation due to leads to histological changes. Gastric injury, pro-inflammatory factors, and oxidative stress in infection produce asymmetric dimethylarginine (ADMA), which are a competitive inhibitor of nitric oxide synthase. Investigations were carried out aimed at finding new drugs derived from natural products for the treatment of . is known to have anti-inflammatory and antimicrobial properties. This study aimed to investigate the effect of extract and a standard eradication regimen on ADMA levels and histological changes in the gastritis rat model.
Thirty-five male rats were randomly and equally divided into five groups. Group-1 was the negative control group and Groups-2 to 5 were -infected groups. Groups-3 to 5 were administered extract, a standard eradication regimen, and a combination of standard eradication regimen and extract, respectively. Histological examination and serum ADMA levels were analyzed. The difference between groups was analyzed using the Kruskal-Wallis and one-way analysis of variance tests. The significance was p<0.05.
Serum ADMA levels and severity of gastritis were higher in infected groups compared to the negative control group (p<0.05). The severity of gastritis and mean ADMA levels in the group that received a single administration of the extract was higher than the others (p<0.05). Serum ADMA levels and severity of gastritis were significantly reduced in the group that received a combination of standard eradication regimen and extract (p<0.05).
Single administration of extract worsens the severity of gastritis and increased serum ADMA levels in the gastritis rat model. The administration of a combination of standard eradication regimen and extract reduces serum ADMA levels and significantly improves the severity of gastritis rat model.
由……导致的胃黏膜或黏膜下层炎症会引发组织学变化。感染中的胃损伤、促炎因子及氧化应激会产生不对称二甲基精氨酸（ADMA），其为一氧化氮合酶的竞争性抑制剂。开展研究旨在寻找源自天然产物的用于治疗……的新药。已知……具有抗炎和抗菌特性。本研究旨在探究……提取物及标准根除方案对胃炎大鼠模型中ADMA水平及组织学变化的影响。
35只雄性大鼠被随机且等分为五组。第1组为阴性对照组，第2至5组为……感染组。第3至5组分别给予……提取物、标准根除方案以及标准根除方案与……提取物的组合。分析组织学检查结果及血清ADMA水平。采用Kruskal-Wallis检验和单因素方差分析来分析组间差异。显著性水平为p < 0.05。
与阴性对照组相比，感染组的血清ADMA水平及胃炎严重程度更高（p < 0.05）。单次给予……提取物的组中胃炎严重程度及平均ADMA水平高于其他组（p < 0.05）。接受标准根除方案与……提取物组合的组中血清ADMA水平及胃炎严重程度显著降低（p < 0.05）。
在胃炎大鼠模型中，单次给予……提取物会加重胃炎严重程度并升高血清ADMA水平。标准根除方案与……提取物联合给药可降低血清ADMA水平并显著改善胃炎大鼠模型的严重程度。
