Institute of Technology, Estonian University of Life Sciences, Tartu, Estonia.
LBN, University of Montpellier, Montpellier, France.
Int J Nanomedicine. 2020 Sep 24;15:7051-7062. doi: 10.2147/IJN.S258060. eCollection 2020.
Nanotechnology applied to cancer treatment is a growing area of research in nanomedicine with magnetic nanoparticle-mediated anti-cancer drug delivery systems offering least possible side effects. To that end, both structural and chemical properties of commercial cobalt metal nanoparticles were studied using label-free confocal Raman spectroscopy.
Crystal structure and morphology of cobalt nanoparticles were studied by XRD and TEM. Magnetic properties were studied with SQUID and PPMS. Confocal Raman microscopy has high spatial resolution and compositional sensitivity. It, therefore, serves as a label-free tool to trace nanoparticles within cells and investigate the interaction between coating-free cobalt metal nanoparticles and cancer cells. The toxicity of cobalt nanoparticles against human cells was assessed by MTT assay.
Superparamagnetic Co metal nanoparticle uptake by MCF7 and HCT116 cancer cells and DPSC mesenchymal stem cells was investigated by confocal Raman microscopy. The Raman nanoparticle signature also allowed accurate detection of the nanoparticle within the cell without labelling. A rapid uptake of the cobalt nanoparticles followed by rapid apoptosis was observed. Their low cytotoxicity, assessed by means of MTT assay against human embryonic kidney (HEK) cells, makes them promising candidates for the development of targeted therapies. Moreover, under a laser irradiation of 20mW with a wavelength of 532nm, it is possible to bring about local heating leading to combustion of the cobalt metal nanoparticles within cells, whereupon opening new routes for cancer phototherapy.
Label-free confocal Raman spectroscopy enables accurately localizing the Co metal nanoparticles in cellular environments. The interaction between the surfactant-free cobalt metal nanoparticles and cancer cells was investigated. The facile endocytosis in cancer cells shows that these nanoparticles have potential in engendering their apoptosis. This preliminary study demonstrates the feasibility and relevance of cobalt nanomaterials for applications in nanomedicine such as phototherapy, hyperthermia or stem cell delivery.
将纳米技术应用于癌症治疗是纳米医学中一个不断发展的研究领域，其中磁性纳米粒子介导的抗癌药物输送系统具有最小的副作用。为此，使用无标记共焦拉曼光谱研究了商业钴金属纳米粒子的结构和化学性质。
通过 XRD 和 TEM 研究了钴纳米粒子的晶体结构和形态。使用 SQUID 和 PPMS 研究了磁性。共焦拉曼显微镜具有高空间分辨率和组成灵敏度。因此，它可作为一种无标记工具，用于跟踪细胞内的纳米粒子，并研究无涂层钴金属纳米粒子与癌细胞之间的相互作用。通过 MTT 测定评估钴纳米粒子对人细胞的毒性。
通过共焦拉曼显微镜研究了 MCF7 和 HCT116 癌细胞和 DPSC 间充质干细胞对超顺磁 Co 金属纳米粒子的摄取。拉曼纳米粒子特征还允许在不标记的情况下准确检测细胞内的纳米粒子。观察到钴纳米粒子的快速摄取，随后迅速凋亡。通过 MTT 测定对人胚肾 (HEK) 细胞的低细胞毒性评估，使它们成为开发靶向治疗的有前途的候选物。此外，在波长为 532nm、功率为 20mW 的激光照射下，可以引起局部加热，导致细胞内的钴金属纳米粒子燃烧，从而为癌症光疗开辟新途径。
无标记共焦拉曼光谱能够准确地将 Co 金属纳米粒子定位在细胞环境中。研究了无表面活性剂的钴金属纳米粒子与癌细胞之间的相互作用。在癌细胞中的易于内吞作用表明这些纳米粒子在诱导其凋亡方面具有潜力。这项初步研究证明了钴纳米材料在光疗、热疗或干细胞输送等纳米医学应用中的可行性和相关性。
