• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

敲低PVT1通过调控miR-106b-5p/FJX1轴抑制结直肠癌进展。

Knockdown of PVT1 Suppresses Colorectal Cancer Progression by Regulating MiR-106b-5p/FJX1 Axis.

作者信息

Liu Fang, Wu Rong, Guan Lina, Tang Xuegui

机构信息

Anorectal Department of Integrated Traditional Chinese and Western Medicine, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, People's Republic of China.

Department of Clinical Medicine of Combination of Chinese and Western Medicine, North Sichuan Medical College, Nanchong, Sichuan, People's Republic of China.

出版信息

Cancer Manag Res. 2020 Sep 22;12:8773-8785. doi: 10.2147/CMAR.S260537. eCollection 2020.

DOI:10.2147/CMAR.S260537
PMID:33061574
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7520100/
Abstract

PURPOSE

Long non-coding RNA plasmacytoma variant translocation 1 (PVT1) has been revealed to involve in the progression of CRC. However, the precise mechanisms of PVT1 in action remain unclear.

METHODS

The expression of PVT1, microRNA-106b-5p (miR-106b-5p) and four jointed box 1 () was measured using quantitative real-time polymerase chain reaction (qRT-PCR) or Western blot, respectively. Cell proliferation was investigated by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide assay. Transwell assay was used to determine cell migration and invasion. The correlation between miR-106b-5p and PVT1 or was confirmed using luciferase reporter assay. The effects of PVT1 in vivo were assessed using mice xenograft model.

RESULTS

PVT1 was up-regulated in CRC tissues and cell lines, especially in CRC tissues with high-grade, and highly expressed PVT1 predicted worse prognosis. Functional experiments demonstrated that PVT1 deletion inhibited CRC cell proliferation, migration and invasion in vitro and suppressed tumor growth in vivo. MiR-106b-5p was confirmed to be a target of PVT1, and inhibition of miR-106b-5p reversed the inhibitory effects of PVT1 knockdown on CRC cell malignant phenotypes. In addition, we found miR-106b-5p directly targeted , and miR-106b-5p-mediated inhibition on CRC cell proliferation, migration and invasion was attenuated by up-regulation. Importantly, it was also proved that PVT1 could indirectly regulate expression via targeting miR-106b-5p.

CONCLUSION

Knockdown of PVT1 impaired cell proliferation, migration and invasion in CRCs via regulating miR-106b-5p/ axis, which provided a novel insight into the development of therapeutic strategies for CRC patients.

摘要

目的

长链非编码RNA浆细胞瘤变异易位1（PVT1）已被揭示参与结直肠癌（CRC）的进展。然而，PVT1发挥作用的确切机制仍不清楚。

方法

分别使用定量实时聚合酶链反应（qRT-PCR）或蛋白质免疫印迹法检测PVT1、微小RNA-106b-5p（miR-106b-5p）和四联体盒1（）的表达。通过3-（4,5）-二甲基噻唑-2,5-二苯基四氮唑溴盐（MTT）法研究细胞增殖。采用Transwell实验测定细胞迁移和侵袭能力。使用荧光素酶报告基因实验证实miR-106b-5p与PVT1或之间的相关性。使用小鼠异种移植模型评估PVT1在体内的作用。

结果

PVT1在CRC组织和细胞系中上调，尤其是在高级别CRC组织中，且PVT1高表达预示着预后较差。功能实验表明，PVT1缺失在体外抑制CRC细胞增殖、迁移和侵袭，并在体内抑制肿瘤生长。miR-106b-5p被证实是PVT1的靶标，抑制miR-106b-5p可逆转PVT1敲低对CRC细胞恶性表型的抑制作用。此外，我们发现miR-106b-5p直接靶向，上调可减弱miR-106b-5p介导的对CRC细胞增殖、迁移和侵袭的抑制作用。重要的是，还证明了PVT1可通过靶向miR-106b-5p间接调节的表达。

结论

敲低PVT1通过调节miR-106b-5p/轴损害CRC细胞的增殖、迁移和侵袭，这为CRC患者治疗策略的开发提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dfc/7520100/dfd4e8e2caf6/CMAR-12-8773-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dfc/7520100/07fa1b03eaea/CMAR-12-8773-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dfc/7520100/fbf9deda737a/CMAR-12-8773-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dfc/7520100/aa7758c5fd9c/CMAR-12-8773-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dfc/7520100/14833dd314ce/CMAR-12-8773-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dfc/7520100/d09cdb07cbe8/CMAR-12-8773-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dfc/7520100/eca6e3cdba6e/CMAR-12-8773-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dfc/7520100/f5e1af92e9a8/CMAR-12-8773-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dfc/7520100/dfd4e8e2caf6/CMAR-12-8773-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dfc/7520100/07fa1b03eaea/CMAR-12-8773-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dfc/7520100/fbf9deda737a/CMAR-12-8773-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dfc/7520100/aa7758c5fd9c/CMAR-12-8773-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dfc/7520100/14833dd314ce/CMAR-12-8773-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dfc/7520100/d09cdb07cbe8/CMAR-12-8773-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dfc/7520100/eca6e3cdba6e/CMAR-12-8773-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dfc/7520100/f5e1af92e9a8/CMAR-12-8773-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dfc/7520100/dfd4e8e2caf6/CMAR-12-8773-g0008.jpg

相似文献

1
Knockdown of PVT1 Suppresses Colorectal Cancer Progression by Regulating MiR-106b-5p/FJX1 Axis.敲低PVT1通过调控miR-106b-5p/FJX1轴抑制结直肠癌进展。
Cancer Manag Res. 2020 Sep 22;12:8773-8785. doi: 10.2147/CMAR.S260537. eCollection 2020.
2
The long non-coding RNA PVT1/miR-145-5p/ITGB8 axis regulates cell proliferation, apoptosis, migration and invasion in non-small cell lung cancer cells.长链非编码 RNA PVT1/miR-145-5p/ITGB8 轴调节非小细胞肺癌细胞的增殖、凋亡、迁移和侵袭。
Neoplasma. 2020 Jul;67(4):802-812. doi: 10.4149/neo_2020_190723N657. Epub 2020 Mar 24.
3
Circ-PVT1/miR-106a-5p/HK2 axis regulates cell growth, metastasis and glycolytic metabolism of oral squamous cell carcinoma.环状 RNA-PVT1/miR-106a-5p/己糖激酶 2 轴调控口腔鳞状细胞癌细胞的生长、转移和糖酵解代谢。
Mol Cell Biochem. 2020 Nov;474(1-2):147-158. doi: 10.1007/s11010-020-03840-5. Epub 2020 Aug 1.
4
Exosome-Derived Circ-PVT1 Contributes to Cisplatin Resistance by Regulating Autophagy, Invasion, and Apoptosis Via miR-30a-5p/YAP1 Axis in Gastric Cancer Cells.外泌体来源的 Circ-PVT1 通过 miR-30a-5p/YAP1 轴调控自噬、侵袭和凋亡促进胃癌细胞顺铂耐药。
Cancer Biother Radiopharm. 2021 May;36(4):347-359. doi: 10.1089/cbr.2020.3578. Epub 2020 Aug 14.
5
Downregulation of miR-216a-5p by long noncoding RNA PVT1 suppresses colorectal cancer progression via modulation of YBX1 expression.长链非编码RNA PVT1对miR-216a-5p的下调通过调节YBX1表达抑制结直肠癌进展。
Cancer Manag Res. 2019 Jul 24;11:6981-6993. doi: 10.2147/CMAR.S208983. eCollection 2019.
6
miR-106b-5p inhibits the invasion and metastasis of colorectal cancer by targeting CTSA.微小RNA-106b-5p通过靶向组织蛋白酶A抑制结直肠癌的侵袭和转移。
Onco Targets Ther. 2018 Jul 4;11:3835-3845. doi: 10.2147/OTT.S172887. eCollection 2018.
7
Long Noncoding RNA Plasmacytoma Variant Translocation 1 Regulates Cisplatin Resistance via miR-3619-5p/TBL1XR1 Axis in Gastric Cancer.长链非编码 RNA 浆细胞瘤变异易位 1 通过 miR-3619-5p/TBL1XR1 轴调控胃癌顺铂耐药。
Cancer Biother Radiopharm. 2020 Dec;35(10):741-752. doi: 10.1089/cbr.2019.3342. Epub 2020 May 14.
8
Knockdown of lncRNA PVT1 inhibits prostate cancer progression in vitro and in vivo by the suppression of KIF23 through stimulating miR-15a-5p.lncRNA PVT1的敲低通过刺激miR-15a-5p抑制KIF23，从而在体外和体内抑制前列腺癌进展。
Cancer Cell Int. 2020 Jul 2;20:283. doi: 10.1186/s12935-020-01363-z. eCollection 2020.
9
MiR-106b-5p regulates the migration and invasion of colorectal cancer cells by targeting FAT4.miR-106b-5p 通过靶向 FAT4 调节结直肠癌细胞的迁移和侵袭。
Biosci Rep. 2020 Nov 27;40(11). doi: 10.1042/BSR20200098.
10
Long non-coding RNA PVT1 regulates glioma proliferation, invasion, and aerobic glycolysis via miR-140-5p.长链非编码 RNA PVT1 通过 miR-140-5p 调控胶质瘤的增殖、侵袭和有氧糖酵解。
Eur Rev Med Pharmacol Sci. 2020 Jan;24(1):274-283. doi: 10.26355/eurrev_202001_19922.

引用本文的文献

1
promotes silica-induced epithelial-mesenchymal transition by modulating the miR-497-5p/TCF3 axis.通过调节miR-497-5p/TCF3轴促进二氧化硅诱导的上皮-间质转化。
J Biomed Res. 2024 Mar 26;38(2):163-174. doi: 10.7555/JBR.37.20220249.
2
Regulation of secretory pathway kinase or kinase-like proteins in human cancers.人类癌症中分泌途径激酶或激酶样蛋白的调节。
Front Immunol. 2023 Feb 7;14:942849. doi: 10.3389/fimmu.2023.942849. eCollection 2023.
3
lncRNA PVT1: a novel oncogene in multiple cancers.长链非编码 RNA PVT1：多种癌症中的新型癌基因。

本文引用的文献

1
An Oncogenic Role for Four-Jointed Box 1 (FJX1) in Nasopharyngeal Carcinoma.FJX1 在鼻咽癌中的致癌作用。
Dis Markers. 2019 May 19;2019:3857853. doi: 10.1155/2019/3857853. eCollection 2019.
2
Amplification of lncRNA PVT1 promotes ovarian cancer proliferation by binding to miR-140.长链非编码 RNA PVT1 通过与 miR-140 结合促进卵巢癌细胞增殖。
Mamm Genome. 2019 Aug;30(7-8):217-225. doi: 10.1007/s00335-019-09808-1. Epub 2019 Jun 20.
3
Circular PVT1: an oncogenic non-coding RNA with emerging clinical importance.环状 PVT1：一种具有新兴临床重要性的致癌性非编码 RNA。
Cell Mol Biol Lett. 2022 Oct 4;27(1):84. doi: 10.1186/s11658-022-00385-x.
4
and spliced transcripts play an oncogenic role in colorectal carcinogenesis.并且剪接转录本在结直肠癌发生过程中发挥致癌作用。
Front Oncol. 2022 Aug 16;12:954634. doi: 10.3389/fonc.2022.954634. eCollection 2022.
5
Long non-coding RNA PVT1: A promising chemotherapy and radiotherapy sensitizer.长链非编码RNA PVT1：一种有前景的化疗和放疗增敏剂。
Front Oncol. 2022 Jul 29;12:959208. doi: 10.3389/fonc.2022.959208. eCollection 2022.
6
Multi-Omics Analysis of the Tumor Microenvironment in Liver Metastasis of Colorectal Cancer Identified FJX1 as a Novel Biomarker.结直肠癌肝转移肿瘤微环境的多组学分析确定FJX1为一种新型生物标志物。
Front Genet. 2022 Jul 19;13:960954. doi: 10.3389/fgene.2022.960954. eCollection 2022.
7
FJX1 as a candidate diagnostic and prognostic serum biomarker for colorectal cancer.FJX1 作为结直肠癌的候选诊断和预后血清生物标志物。
Clin Transl Oncol. 2022 Oct;24(10):1964-1974. doi: 10.1007/s12094-022-02852-5. Epub 2022 Jun 1.
8
CircPVT1: a pivotal circular node intersecting Long Non-Coding-PVT1 and c-MYC oncogenic signals.环状 RNA PVT1：一个关键的环状节点，交汇长链非编码 RNA PVT1 和 c-MYC 致癌信号。
Mol Cancer. 2022 Jan 28;21(1):33. doi: 10.1186/s12943-022-01514-y.
9
N1, N12-Diacetylspermine Is Elevated in Colorectal Cancer and Promotes Proliferation through the miR-559/CBS Axis in Cancer Cell Lines.N1,N12-二乙酰精胺在结直肠癌中升高，并通过miR-559/CBS轴促进癌细胞系增殖。
J Oncol. 2021 Sep 24;2021:6665704. doi: 10.1155/2021/6665704. eCollection 2021.
10
Exosomal lncRNA PVT1/VEGFA Axis Promotes Colon Cancer Metastasis and Stemness by Downregulation of Tumor Suppressor miR-152-3p.外泌体长链非编码 RNA PVT1/VEGFA 轴通过下调肿瘤抑制 miR-152-3p 促进结肠癌转移和干性。
Oxid Med Cell Longev. 2021 Jul 15;2021:9959807. doi: 10.1155/2021/9959807. eCollection 2021.
J Clin Pathol. 2019 Aug;72(8):513-519. doi: 10.1136/jclinpath-2019-205891. Epub 2019 Jun 1.
4
Knockdown of long noncoding RNA PVT1 suppresses cell proliferation and invasion of colorectal cancer via upregulation of microRNA-214-3p.敲低长链非编码 RNA PVT1 通过上调 microRNA-214-3p 抑制结直肠癌细胞的增殖和侵袭。
Am J Physiol Gastrointest Liver Physiol. 2019 Aug 1;317(2):G222-G232. doi: 10.1152/ajpgi.00357.2018. Epub 2019 May 24.
5
Genomic data mining for functional annotation of human long noncoding RNAs.基因组数据挖掘在人类长非编码 RNA 功能注释中的应用。
J Zhejiang Univ Sci B. 2019 Jun;20(6):476-487. doi: 10.1631/jzus.B1900162.
6
LncRNA PVT1 promotes proliferation, invasion and epithelial-mesenchymal transition of renal cell carcinoma cells through downregulation of miR-16-5p.长链非编码RNA PVT1通过下调miR-16-5p促进肾癌细胞的增殖、侵袭和上皮-间质转化。
Onco Targets Ther. 2019 Apr 5;12:2563-2575. doi: 10.2147/OTT.S190239. eCollection 2019.
7
The prognostic significance of the long non-coding RNAs "CCAT1, PVT1" in t(8;21) associated Acute Myeloid Leukemia.CCAT1、PVT1 长非编码 RNA 在 t(8;21)相关急性髓系白血病中的预后意义。
Gene. 2019 Jul 30;707:172-177. doi: 10.1016/j.gene.2019.03.055. Epub 2019 Mar 31.
8
Long Non-coding RNA PVT1 Competitively Binds MicroRNA-424-5p to Regulate CARM1 in Radiosensitivity of Non-Small-Cell Lung Cancer.长链非编码RNA PVT1通过竞争性结合微小RNA-424-5p来调控CARM1，影响非小细胞肺癌的放射敏感性
Mol Ther Nucleic Acids. 2019 Jun 7;16:130-140. doi: 10.1016/j.omtn.2018.12.006. Epub 2018 Dec 15.
9
MALAT1 sponges miR-106b-5p to promote the invasion and metastasis of colorectal cancer via SLAIN2 enhanced microtubules mobility.MALAT1 通过 SLAIN2 增强微管迁移促进结直肠癌细胞的侵袭和转移，从而形成海绵体 miR-106b-5p。
EBioMedicine. 2019 Mar;41:286-298. doi: 10.1016/j.ebiom.2018.12.049. Epub 2019 Feb 21.
10
Long Noncoding RNA Plasmacytoma Variant Translocation 1 (PVT1) Promotes Colon Cancer Progression via Endogenous Sponging miR-26b.长链非编码 RNA 浆细胞瘤变异易位 1（PVT1）通过内源性海绵吸附 miR-26b 促进结肠癌进展。
Med Sci Monit. 2018 Dec 1;24:8685-8692. doi: 10.12659/MSM.910955.