Department of Health Science University of Milan via A. di Rudinì 8 20142 Milan.
Int J Biol Sci. 2020 Sep 30;16(15):3018-3027. doi: 10.7150/ijbs.49302. eCollection 2020.
S-adenosylmethionine supplies methyl groups to many acceptors, including lipids, proteins, RNA, DNA, and a wide range of small molecules. It acts as the precursor in the biosynthesis of metal ion chelating compounds, such as nicotianamine and phytosiderophores, of the polyamines spermidine and spermine and of some plant hormones. Finally, it is the source of catalytic 5'-deoxyadenosyl radicals. Radical S-adenosylmethionine (SAM) enzymes (RS) represent one of the most abundant groups (more than 100,000) of enzymes, exerting a plethora of biological functions, some of which are still unknown. In this work, we will focus on two RS: CDK5RAP1 and CDKAL1, both of which are involved in tRNA modifications that result in important tRNA folding and stability and in maintaining high translational fidelity. Based on this crucial role, their impairment can be important in the development of different human diseases.
S-腺苷甲硫氨酸向包括脂质、蛋白质、RNA、DNA 和各种小分子在内的许多受体提供甲基。它作为生物合成金属离子螯合物（如尼克酸胺和植物铁载体）、多胺（亚精胺和精胺）和一些植物激素的前体。最后，它是催化 5'-脱氧腺苷自由基的来源。自由基 S-腺苷甲硫氨酸（SAM）酶（RS）是最丰富的酶组之一（超过 100,000 种），发挥着多种生物学功能，其中一些仍未知。在这项工作中，我们将重点关注两种 RS：CDK5RAP1 和 CDKAL1，它们都参与 tRNA 的修饰，导致重要的 tRNA 折叠和稳定性，并维持高翻译保真度。基于这一关键作用，它们的损伤在人类多种疾病的发展中可能很重要。
