Deptuła Piotr, Łysik Dawid, Pogoda Katarzyna, Cieśluk Mateusz, Namiot Andrzej, Mystkowska Joanna, Król Grzegorz, Głuszek Stanisław, Janmey Paul A, Bucki Robert
Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Bialystok, 15-222 Bialystok, Poland.
Institute of Biomedical Engineering, Bialystok University of Technology, 15-351 Bialystok, Poland.
ACS Biomater Sci Eng. 2020 Oct 12;6(10):5620-5631. doi: 10.1021/acsbiomaterials.0c00975. Epub 2020 Sep 7.
In recent years, rheological measurements of cells and tissues at physiological and pathological stages have become an essential method to determine how forces and changes in mechanical properties contribute to disease development and progression, but there is no standardization of this procedure so far. In this study, we evaluate the potential of nanoscale atomic force microscopy (AFM) and macroscopic shear rheometry to assess the mechanical properties of healthy and cancerous human colon tissues. The direct comparison of tissue mechanical behavior under uniaxial and shear deformation shows that cancerous tissues not only are stiffer compared to healthy tissue but also respond differently when shear and compressive stresses are applied. These results suggest that rheological parameters can be useful measures of colon cancer mechanopathology. Additionally, we extend the list of biological materials exhibiting compressional stiffening and shear weakening effects to human colon tumors. These mechanical responses might be promising mechanomarkers and become part of the new procedures in colon cancer diagnosis. Enrichment of histopathological grading with rheological assessment of tissue mechanical properties will potentially allow more accurate colon cancer diagnosis and improve prognosis.
近年来，对处于生理和病理阶段的细胞及组织进行流变学测量已成为确定力和机械性能变化如何影响疾病发展和进程的重要方法，但目前该程序尚无标准化。在本研究中，我们评估了纳米级原子力显微镜（AFM）和宏观剪切流变仪评估健康和癌性人类结肠组织力学性能的潜力。对单轴和剪切变形下组织力学行为的直接比较表明，癌性组织不仅比健康组织更硬，而且在施加剪切应力和压缩应力时反应也不同。这些结果表明，流变学参数可能是结肠癌机械病理学的有用指标。此外，我们将表现出压缩硬化和剪切弱化效应的生物材料列表扩展到人类结肠肿瘤。这些力学反应可能是有前景的机械标志物，并成为结肠癌诊断新程序的一部分。用组织力学性能的流变学评估丰富组织病理学分级可能会使结肠癌诊断更准确并改善预后。
