样本合并以优化 SARS-CoV-2 的 RT-qPCR 诊断：两种方案的比较分析。
Pooling of samples to optimize SARS-CoV-2 diagnosis by RT-qPCR: comparative analysis of two protocols.
机构信息
LABRESIS- Laboratório de Pesquisa em Resistência Bacteriana, Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, RS, Brazil.
Programa de Pós-Graduação em Ciências Médicas (PPGCM), Faculdade de Medicina, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
出版信息
Eur J Clin Microbiol Infect Dis. 2021 Apr;40(4):889-892. doi: 10.1007/s10096-020-04071-8. Epub 2020 Oct 16.
Abstract
This study has aimed to evaluate the use pool of samples as a strategy to optimize the diagnostic of SARS-CoV-2 by RT-qPCR. A total of 220 naso/orofaryngeal swab samples were collected and tested using two different protocols of sample pooling. Results from protocol A were identical with the individual results. However, for results from protocol B, reduced agreement (91%) was observed in relation to individual testing. Inconsistencies observed were related to RT-qPCR results with higher cycle thresholds. These results suggest that pooling of samples before RNA extraction is preferable in terms of diagnostic for SARS-CoV-2.
摘要
本研究旨在评估样本混合池策略在优化 SARS-CoV-2 的 RT-qPCR 诊断中的应用。共采集 220 份鼻咽或口咽拭子样本，使用两种不同的样本混合方案进行检测。方案 A 的结果与个体检测结果完全一致。然而，对于方案 B 的结果，与个体检测相比，一致性降低（91%）。观察到的不一致与 RT-qPCR 结果中较高的循环阈值有关。这些结果表明，在进行 RNA 提取之前对样本进行混合是 SARS-CoV-2 诊断的优选方法。
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2
COVID-19: Epidemiology, Evolution, and Cross-Disciplinary Perspectives.新型冠状病毒肺炎：流行病学、进化与跨学科视角。
Trends Mol Med. 2020 May;26(5):483-495. doi: 10.1016/j.molmed.2020.02.008. Epub 2020 Mar 21.
3
Recent advances and perspectives of nucleic acid detection for coronavirus.冠状病毒核酸检测的最新进展与展望
J Pharm Anal. 2020 Apr;10(2):97-101. doi: 10.1016/j.jpha.2020.02.010. Epub 2020 Mar 1.
4
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J Clin Microbiol. 2020 May 26;58(6). doi: 10.1128/JCM.00512-20.
5
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Micromachines (Basel). 2020 Mar 14;11(3):306. doi: 10.3390/mi11030306.
6
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J Clin Microbiol. 2014 Jan;52(1):67-75. doi: 10.1128/JCM.02533-13. Epub 2013 Oct 23.
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