Han Chao, Wang Ya-Jun, Wang Ya-Chen, Guan Xin, Wang Liang, Shen Li-Ming, Zou Wei, Liu Jing
Stem Cell Clinical Research Center, Regenerative Medicine Center; National Joint Engineering Laboratory, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning Province, China.
College of Life Science, Liaoning Normal University, Dalian, Liaoning Province, China.
Neural Regen Res. 2021 Apr;16(4):714-720. doi: 10.4103/1673-5374.295342.
Previous studies have shown that caveolin-1 is involved in regulating the differentiation of mesenchymal stem cells. However, its role in the differentiation of human adipose mesenchymal stem cells into dopaminergic neurons remains unclear. The aim of this study was to investigate whether caveolin-1 regulates the differentiation of human adipose mesenchymal stem cells into dopaminergic-like neurons. We also examined whether the expression of caveolin-1 could be modulated by RNA interference technology to promote the differentiation of human adipose mesenchymal stem cells into dopaminergic-like neurons. The differentiation of human adipose mesenchymal stem cells into dopaminergic neurons was evaluated morphologically and by examining expression of the markers tyrosine hydroxylase, Lmx1a and Nurr1. The analyses revealed that during the differentiation of human adipose mesenchymal stem cells into dopaminergic neurons, the expression of caveolin-1 is decreased. Notably, the downregulation of caveolin-1 promoted the differentiation of human adipose mesenchymal stem cells into dopaminergic-like neurons, and it increased the expression of tyrosine hydroxylase, Lmx1a and Nurr1. Together, our findings suggest that caveolin-1 plays a negative regulatory role in the differentiation of dopaminergic-like neurons from stem cells, and it may therefore be a potential molecular target for strategies for regulating the differentiation of these cells. This study was approved by the Medical Ethics Committee of the First Affiliated Hospital of Dalian Medical University of China (approval No. PJ-KS-KY-2020-54) on March 7, 2017.
先前的研究表明，小窝蛋白-1参与调节间充质干细胞的分化。然而，其在人脂肪间充质干细胞向多巴胺能神经元分化中的作用仍不清楚。本研究的目的是探讨小窝蛋白-1是否调节人脂肪间充质干细胞向多巴胺能样神经元的分化。我们还研究了是否可以通过RNA干扰技术调节小窝蛋白-1的表达，以促进人脂肪间充质干细胞向多巴胺能样神经元的分化。通过形态学评估以及检测酪氨酸羟化酶、Lmx1a和Nurr1等标志物的表达，来评价人脂肪间充质干细胞向多巴胺能神经元的分化情况。分析显示，在人脂肪间充质干细胞向多巴胺能神经元分化过程中，小窝蛋白-1的表达降低。值得注意的是，小窝蛋白-1的下调促进了人脂肪间充质干细胞向多巴胺能样神经元的分化，并增加了酪氨酸羟化酶、Lmx1a和Nurr1的表达。总之，我们的研究结果表明，小窝蛋白-1在干细胞向多巴胺能样神经元的分化中起负调节作用，因此它可能是调节这些细胞分化策略的潜在分子靶点。本研究于2017年3月7日获得中国大连医科大学附属第一医院医学伦理委员会批准（批准号：PJ-KS-KY-2020-54）。
