Microbiology Division, Department of Pathology, Faculty of Medicine, University of Porto - Alameda Prof. Hernani Monteiro, Porto, Portugal.
CAPES Foundation, Ministry of Education of Brazil, Brasília, Brazil.
Med Vet Entomol. 2021 Jun;35(2):225-229. doi: 10.1111/mve.12486. Epub 2020 Oct 16.
Antibiotic-resistant bacteria pose a major threat to global health in the 21st century, requiring a quick, cheap and effective response from public health officials. This study evaluated the antimicrobial activity of native excretions/secretions (NES) produced by third instar (3 days old) larvae of Calliphora vicina using a protocol adapted from the Institute of Clinical and Laboratory Standards (CLSI). The microorganisms tested were: Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and the fungus Candida albicans. After the incubation period, the suspensions were diluted and spread on nutrient agar plates to count the colony-forming units. A turbidimetric test also was carried out to test the action of the NES of C. vicina against S. aureus, a very common bacterial species, with an enormous capacity for adaption and resistance, being one of the bacteria of medical importance that causes the most hospital and community infections in the world. According to our results, the NES of C. vicina exhibits antimicrobial activity at different dilutions, being most effective against the gram-negative bacteria E. coli and K. pneumoniae.
在 21 世纪，抗药性细菌对全球健康构成重大威胁，这需要公共卫生官员迅速、廉价且有效地做出反应。本研究使用改编自临床和实验室标准研究所 (CLSI) 的方案，评估了第三龄（3 日龄）丽蝇蛹集金小蜂幼虫产生的天然分泌物/分泌物 (NES) 的抗菌活性。测试的微生物包括：大肠杆菌、肺炎克雷伯菌、金黄色葡萄球菌和真菌白色念珠菌。孵育期结束后，将悬浮液稀释并铺在营养琼脂平板上，以计算形成菌落的单位数。还进行了浊度试验，以测试丽蝇蛹集金小蜂 NES 对金黄色葡萄球菌的作用，金黄色葡萄球菌是一种非常常见的细菌，具有极强的适应和耐药能力，是世界上导致医院和社区感染最多的重要医学细菌之一。根据我们的结果，丽蝇蛹集金小蜂的 NES 在不同稀释度下表现出抗菌活性，对革兰氏阴性菌大肠杆菌和肺炎克雷伯菌最有效。
