Division of Oral Diagnosis, Department of Diagnostic and Therapeutic Science, Meikai University School of Dentistry, Sakado, Saitama, Japan.
In Vivo. 2012 Sep-Oct;26(5):759-64.
The purpose of this study was to evaluate the histopathological effects of curcumin and capsaicin, with or without visible light (VL) irradiation for 5 min, on the oral mucous membrane in mice. Capsaicin-treated, but not curcumin-treated, buccal epithelium exhibited slight tissue damage; VL irradiation caused excessive tissue damage, particularly when combined with the former treatment. The TdT-mediated dUTP-biotin nick end-labeling (TUNEL) method demonstrated that both capsaicin and curcumin induced apoptosis, with the apoptotic effect of capsaicin appearing at an early stage of application. VL irradiation increased the number of apoptotic cells, particularly those upon in the capsaicin-treated area. Capsaicin and curcumin acted as photosensitizers exposure to VL, in the presence of oxygen. Curcumin and capsaicin with VL irradiation could thus be used for photodynamic therapy in the clinical setting, especially in precancerous oral diseases.
本研究旨在评估姜黄素和辣椒素在可见光（VL）照射 5 分钟的情况下对口腔黏膜的组织病理学影响。与姜黄素处理不同，辣椒素处理的颊上皮仅表现出轻微的组织损伤；VL 照射会导致过度的组织损伤，特别是与前者联合处理时。末端转移酶介导的 dUTP-生物素缺口末端标记（TUNEL）法表明，姜黄素和辣椒素均可诱导细胞凋亡，且辣椒素的凋亡作用出现在早期应用阶段。VL 照射增加了细胞凋亡的数量，尤其是在辣椒素处理区域。姜黄素和辣椒素在氧气存在下作为光敏剂，暴露于 VL 时会发生反应。因此，姜黄素和辣椒素与 VL 照射可用于临床光动力疗法，特别是在癌前口腔疾病中。
