基于长波长 TCF 的荧光探针用于生物硫醇的检测和细胞内成像。
Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols.
机构信息
Department of Chemistry, University of Bath, Bath, BA2 7AY, UK.
出版信息
Chem Commun (Camb). 2018 May 8;54(38):4786-4789. doi: 10.1039/c8cc01661e.
Two 'turn on' TCF-based fluorescence probes were developed for the detection of biological thiols (TCF-GSH and TCFCl-GSH). TCF-GSH was shown to have a high sensitivity towards glutathione (GSH) with a 0.28 μM limit of detection. Unfortunately, at higher GSH concentrations the fluorescence intensity of TCF-GSH decreased and toxicity was observed for TCF-GSH in live cells. However, TCFCl-GSH was shown to be able to detect GSH at biologically relevant concentrations with a 0.45 μM limit of detection. No toxicity was found for TCFCl-GSH and a clear 'turn on' with good photostability was observed for the exogenous addition of GSH, Cys and HCys. Furthermore, TCFCl-GSH was used to evaluate the effects of drug treatment on the levels of GSH in live cells.
两种基于 TCF 的“开启”荧光探针被开发用于检测生物硫醇（TCF-GSH 和 TCFCl-GSH）。TCF-GSH 对谷胱甘肽（GSH）具有高灵敏度，检测限为 0.28 μM。不幸的是，在较高的 GSH 浓度下，TCF-GSH 的荧光强度降低，并且在活细胞中观察到 TCF-GSH 的毒性。然而，TCFCl-GSH 被证明能够以 0.45 μM 的检测限在生物相关浓度下检测 GSH。TCFCl-GSH 未发现毒性，并且在外源性添加 GSH、Cys 和 HCys 时观察到明显的“开启”和良好的光稳定性。此外，TCFCl-GSH 用于评估药物处理对活细胞中 GSH 水平的影响。
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