Hietanen J, Pihlman K, Förström L, Linder E, Reunala T
Scand J Dent Res. 1987 Aug;95(4):320-7. doi: 10.1111/j.1600-0722.1987.tb01848.x.
Twelve patients with oral lichen planus (OLP) suspected of dental restorative metal allergy were examined. All patients were patch tested with several metals including six different mercury compounds. One (8%) patient showed a positive patch test to two mercury compounds whereas no mercury allergy was found in a reference group of 17 patients suspected of dental restorative material allergy but without OLP lesions. The mercury allergic OLP patient was further tested on palatal mucosa but no reactions developed. Moreover, the energy dispersive X-ray microanalysis failed to show any contaminating metals in his OLP lesion. Mucosal biopsies were taken in close contact with amalgam fillings from nine OLP patients but these disclosed no evidence of lichenoid or dysplastic alterations. In OLP lesions, the immunofluorescence findings showed fibrinogen deposition, altered basement membrane and elastic fiber staining and intense Ulex europeaus I lectin fluorescence through all epithelial cell layers. Therefore, the present patch tests did not reveal increased frequency of mercury or other metal allergy in OLP patients and the mucosal biopsies failed to show any histologic or immunofluorescence alterations deviating from idiopathic OLP lesions.
对12例怀疑对牙科修复金属过敏的口腔扁平苔藓（OLP）患者进行了检查。所有患者均用包括六种不同汞化合物在内的几种金属进行了斑贴试验。1例（8%）患者对两种汞化合物斑贴试验呈阳性，而在17例怀疑对牙科修复材料过敏但无OLP损害的对照患者中未发现汞过敏。对汞过敏的OLP患者进一步在腭黏膜上进行试验，但未出现反应。此外，能量色散X射线微分析未能在其OLP损害中显示任何污染金属。从9例OLP患者与汞合金充填物紧密接触处取黏膜活检，但未发现苔藓样或发育异常改变的证据。在OLP损害中，免疫荧光检查结果显示纤维蛋白原沉积、基底膜和弹性纤维染色改变，以及贯穿所有上皮细胞层的强烈欧洲荆豆I凝集素荧光。因此，目前的斑贴试验未显示OLP患者中汞或其他金属过敏的频率增加，黏膜活检也未显示任何与特发性OLP损害不同的组织学或免疫荧光改变。
