Laboratory of Natural Medicine Research and Development in Wuling Mountain, School of Chemistry and Chemical Engineering, Yangtze Normal University, Fuling 408100, People's Republic of China.
Chongqing Traditional Chinese Medicine Hospital, Chongqing 400021, People's Republic of China; State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, People's Republic of China.
Fitoterapia. 2020 Nov;147:104755. doi: 10.1016/j.fitote.2020.104755. Epub 2020 Oct 16.
Hypersubones D-H (1-5), five new polycyclic polyprenylated acylphloroglucinols (PPAPs) type metabolites with intriguing adamantane and homo-adamantane skeletons, were characterized from aerial parts of Hypericum subsessile. Compounds 1-2 were elucidated to share an adamantane core with 28,29-expoxide moiety, while 3-5 were homo-adamantane type PPAPs sharing a1,2-dioxepane ring system. Their structures were determined on the basis of comprehensive NMR and MS spectroscopic data.The anti-adipogenesis activities of these isolates were evaluated through employing 3T3-L1 cells as an in vitro system using oil red O staining, and compounds 1, 2 and 5 were able to significant inhibit the adipocyte differentiation, which implied that these compounds possessed anti-adipogenic activity.
从无柄金丝桃的地上部分分离得到 5 个新的多环多聚异戊烯基酰基间苯三酚（PPAP）类代谢物 Hypersubones D-H（1-5），它们具有有趣的金刚烷和同金刚烷骨架。化合物 1-2 被阐明为具有 28,29-环氧部分的金刚烷核心，而 3-5 为同金刚烷型 PPAP，共享 1,2-二氧杂环戊烷环系统。基于全面的 NMR 和 MS 光谱数据确定了它们的结构。通过使用油红 O 染色，采用 3T3-L1 细胞作为体外系统，评估了这些分离物的抗脂肪生成活性，化合物 1、2 和 5 能够显著抑制脂肪细胞分化，这表明这些化合物具有抗脂肪生成活性。
