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后基因组时代可持续农业中的植物-微生物相互作用

Plant-microbe Interactions for Sustainable Agriculture in the Post-genomic Era.

作者信息

Agrahari Raj Kishan, Singh Prashantee, Koyama Hiroyuki, Panda Sanjib Kumar

机构信息

1Faculty of Applied Biological Sciences, Gifu University, Gifu501-1193, Japan; 2Lab of Plant-Microbe Interactions, Centre for DNA Fingerprinting and Diagnostics, Graduate studies: Manipal Academy of Higher Education, Manipal, Karnataka, India; 3Department of Biochemistry, School of Life Sciences, Central University of Rajasthan, Rajasthan 305817, India.

出版信息

Curr Genomics. 2020 Apr;21(3):168-178. doi: 10.2174/1389202921999200505082116.

Abstract

Plant-microbe interactions are both symbiotic and antagonistic, and the knowledge of both these interactions is equally important for the progress of agricultural practice and produce. This review gives an insight into the recent advances that have been made in the plant-microbe interaction study in the post-genomic era and the application of those for enhancing agricultural production. Adoption of next-generation sequencing (NGS) and marker assisted selection of resistant genes in plants, equipped with cloning and recombination techniques, has progressed the techniques for the development of resistant plant varieties by leaps and bounds. Genome-wide association studies (GWAS) of both plants and microbes have made the selection of desirable traits in plants and manipulation of the genomes of both plants and microbes effortless and less time-consuming. Stress tolerance in plants has been shown to be accentuated by association of certain microorganisms with the plant, the study and application of the same have helped develop stress-resistant varieties of crops. Beneficial microbes associated with plants are being extensively used for the development of microbial consortia that can be applied directly to the plants or the soil. Next-generation sequencing approaches have made it possible to identify the function of microbes associated in the plant microbiome that are both culturable and non-culturable, thus opening up new doors and possibilities for the use of these huge resources of microbes that can have a potential impact on agriculture.

摘要

植物与微生物的相互作用既有共生的一面,也有拮抗的一面,了解这两种相互作用对于农业实践和农产品的发展同样重要。本综述深入探讨了后基因组时代植物与微生物相互作用研究的最新进展,以及这些进展在提高农业产量方面的应用。采用下一代测序(NGS)和植物抗性基因的标记辅助选择,并配备克隆和重组技术,极大地推动了抗性植物品种的开发技术。植物和微生物的全基因组关联研究(GWAS)使植物中理想性状的选择以及植物和微生物基因组的操作变得轻松且耗时更少。已表明某些微生物与植物的关联可增强植物的胁迫耐受性,对其进行的研究和应用有助于培育抗逆作物品种。与植物相关的有益微生物正被广泛用于开发可直接应用于植物或土壤的微生物群落。下一代测序方法使鉴定植物微生物组中可培养和不可培养的相关微生物的功能成为可能,从而为利用这些可能对农业产生潜在影响的大量微生物资源打开了新的大门和可能性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57af/7521031/7758398c2e85/CG-21-168_F1.jpg

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