Lachén-Montes Mercedes, Corrales Fernando J, Fernández-Irigoyen Joaquín, Santamaría Enrique
Clinical Neuroproteomics Unit, Proteomics Platform, Navarrabiomed, Complejo Hospitalario de Navarra (CHN), Universidad Pública de Navarra (UPNA), Pamplona, Spain.
IdiSNA, Navarra Institute for Health Research, Pamplona, Spain.
Front Microbiol. 2020 Sep 22;11:2101. doi: 10.3389/fmicb.2020.02101. eCollection 2020.
Like other RNA viruses, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replicates in host cells, continuously modulating the molecular environment. It encodes 28 multifunctional proteins that induce an imbalance in the metabolic and proteostatic homeostasis in infected cells. Recently, proteomic approaches have allowed the evaluation of the impact of SARS-CoV-2 infection in human cells. Here, we discuss the current use of proteomics in three major application areas: (i) virus-protein interactomics, (ii) differential proteotyping to map the virus-induced changes in different cell types, and (iii) diagnostic methods for coronavirus infectious disease 2019 (COVID-19). Since the nasal cavity is one of the entry sites for SARS-CoV-2, we will also discuss the potential application of olfactory proteomics to provide novel insights into the olfactory dysfunction triggered by SARS-CoV-2 in patients with COVID-19.
与其他RNA病毒一样，严重急性呼吸综合征冠状病毒2（SARS-CoV-2）在宿主细胞中复制，不断调节分子环境。它编码28种多功能蛋白，这些蛋白会导致受感染细胞的代谢和蛋白质稳态失衡。最近，蛋白质组学方法已能够评估SARS-CoV-2感染对人类细胞的影响。在此，我们讨论蛋白质组学在三个主要应用领域的当前用途：（i）病毒-蛋白质相互作用组学，（ii）差异蛋白质分型以绘制病毒在不同细胞类型中引起的变化，以及（iii）2019冠状病毒病（COVID-19）的诊断方法。由于鼻腔是SARS-CoV-2的进入部位之一，我们还将讨论嗅觉蛋白质组学的潜在应用，以提供关于COVID-19患者中由SARS-CoV-2引发的嗅觉功能障碍的新见解。
