Shen Fengge, Ge Chunpo, Yuan Peng
Xinxiang Key Laboratory of Molecular Neurology, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, China.
School of Public Health, Xinxiang Medical University, Xinxiang, China.
Front Microbiol. 2020 Sep 18;11:538572. doi: 10.3389/fmicb.2020.538572. eCollection 2020.
() is a global health threat accompanied by increasing in drug resistance. To combat this challenge, there is an urgent need to find alternative antimicrobial agents against . This study investigated the antimicrobial efficacy of carnosol against using an model. The effects of carnosol were determined based on the antimicrobial effects or formation and disruption of biofilms. Finally, metabolomics of grown as planktonic cells and biofilms with carnosol treatment were analyzed using gas chromatography-mass spectrometry. The minimum inhibitory concentrations (MICs) of carnosol were 32 to 256 μg/mL against the sixteen tested strains. Among the biofilms, we observed a reduction in bacterial motility of the , biofilm development and preformed biofilm after carnosol treatment. Moreover, the significantly altered metabolic pathways upon carnosol treatment in planktonic cells and biofilms were highly associated with the perturbation of glyoxylate and dicarboxylate metabolism, glycine, serine and threonine metabolism, arginine and proline metabolism, alanine, aspartate and glutamate metabolism, arginine biosynthesis, and aminoacyl-tRNA biosynthesis. In addition, glutathione metabolism, D-glutamine and D-glutamate metabolism were significantly changed in the biofilms. This study establishes the antibacterial and antibiofilm properties of carnosol, and will provide an alternative strategy for overcoming the drug resistance of .
（）是一种伴随着耐药性增加的全球健康威胁。为应对这一挑战，迫切需要找到针对（）的替代抗菌剂。本研究使用（）模型研究了鼠尾草酸对（）的抗菌效果。基于抗菌效果或生物膜的形成与破坏来确定鼠尾草酸的作用。最后，使用气相色谱 - 质谱联用技术分析了经鼠尾草酸处理的浮游细胞和生物膜中（）的代谢组学。鼠尾草酸对16株受试（）菌株的最低抑菌浓度（MIC）为32至256μg/mL。在生物膜中，我们观察到经鼠尾草酸处理后（）的细菌运动性降低、生物膜形成减少以及成熟生物膜受到破坏。此外，鼠尾草酸处理后（）浮游细胞和生物膜中显著改变的代谢途径与乙醛酸和二羧酸代谢、甘氨酸、丝氨酸和苏氨酸代谢、精氨酸和脯氨酸代谢、丙氨酸、天冬氨酸和谷氨酸代谢、精氨酸生物合成以及氨酰 - tRNA生物合成的扰动高度相关。此外，生物膜中的谷胱甘肽代谢、D - 谷氨酰胺和D - 谷氨酸代谢也发生了显著变化。本研究确定了鼠尾草酸的抗菌和抗生物膜特性，并将为克服（）的耐药性提供一种替代策略。
