Department of Microbiology, Faculty of Natural Sciences, Siedlce University of Natural Sciences and Humanities, 14 Bolesława Prusa Str., 08-110 Siedlce, Poland.
Department of Biochemistry and Molecular Biology, Faculty of Natural Sciences, Siedlce University of Natural Sciences and Humanities, 14 Bolesława Prusa Str., 08-110 Siedlce, Poland.
Int J Mol Sci. 2018 Nov 6;19(11):3487. doi: 10.3390/ijms19113487.
The role of genes that are essential for development of biofilm during infection is not fully known. mRNA from two methicillin-resistant strains that formed weak and strong biofilm on polystyrene plates were isolated at five time points from cells grown in biofilm and planktonic culture. Quantitative real-time PCR analysis showed that the expression levels of investigated genes under biofilm conditions were significantly higher than under planktonic conditions. The expression levels of the gene encoding elastin binding protein () and laminin binding protein () were significantly increased in biofilm at 3 h, both in strongly and weakly adhering strain. The peak expression of gene encoding fibrinogen binding protein was found at 6 and 8 h in the case of strongly and weakly adhering strain, respectively. The expression of and genes in both strains was significantly higher under biofilm conditions when comparing to planktonic cells during 12 h. The expression level of the genes encoding binding proteins and the glucosamine polymer polysaccharide intercellular adhesin (PIA) slowly decreased after 24 h. Finally, we found that the expression levels of genes encoding binding factors in weakly adhering strain were significantly lower than in strongly adhering strain.
在感染过程中，对于生物膜形成至关重要的基因的作用尚不完全清楚。从在聚苯乙烯板上形成弱和强生物膜的两种耐甲氧西林的菌株的细胞中分离在生物膜和浮游培养物中生长的五个时间点的 mRNA。实时定量 PCR 分析表明，在生物膜条件下，调查基因的表达水平明显高于浮游条件。在 3 小时时，弹性蛋白结合蛋白（）和层粘连蛋白结合蛋白（）的编码基因的表达水平在强和弱附着菌株中均显著增加。在强和弱附着菌株中，纤维蛋白原结合蛋白的编码基因的表达峰值分别出现在 6 和 8 小时。与浮游细胞相比，两种菌株在 12 小时的生物膜条件下，基因的表达水平明显更高。在 24 小时后，结合蛋白编码基因和葡萄糖胺聚合物细胞间黏附素（PIA）的表达水平缓慢下降。最后，我们发现弱附着菌株中结合因子编码基因的表达水平明显低于强附着菌株。
