Rand S, Ritter P, Kohfahl A, Brinkmann B
Z Rechtsmed. 1987;98(3):175-80. doi: 10.1007/BF00200340.
A comparison of separation and detection techniques has been carried out to determine the most suitable combination for use in Gc-subtyping. The best results (i.e., high sensitivity, distinct bands, especially with reference to the 1S and 1F separation) were achieved using isoelectric focusing in polyacrylamide gel (pH 4.5-5.4) followed by transfer to nitrocellulose membrane by electroblotting and finally detection with enzyme-linked antibody complex.
为了确定用于Gc亚型分型的最合适组合，对分离和检测技术进行了比较。使用聚丙烯酰胺凝胶（pH 4.5 - 5.4）中的等电聚焦，然后通过电印迹转移到硝酸纤维素膜上，最后用酶联抗体复合物进行检测，获得了最佳结果（即高灵敏度、清晰的条带，特别是对于1S和1F的分离）。
