Université Côte d'Azur, CNRS, INSERM, Institute for Research on Cancer and Aging, Nice (IRCAN), Nice, France.
Methods Mol Biol. 2021;2219:69-80. doi: 10.1007/978-1-0716-0974-3_4.
Animal regeneration is a biological process leading to the reformation of injured or lost tissues/body parts. One of the most fascinating regenerative phenomena is the so-called whole-body regeneration, leading to the reformation of fully functional organisms within days after bisection. The sea anemone Nematostella vectensis is currently emerging as novel whole-body regeneration model. Here we describe the methods of inducing the regenerative process in this cnidarian as well as the fixation and staining protocols for morphological, molecular, and cellular analysis.
动物再生是一种导致受伤或失去的组织/身体部位重新形成的生物学过程。最引人注目的再生现象之一是所谓的全身再生，它导致生物体在二分法后几天内重新形成具有完全功能的个体。海葵 Nematostella vectensis 目前作为一种新型的全身再生模型出现。在这里，我们描述了在这种刺胞动物中诱导再生过程的方法，以及用于形态、分子和细胞分析的固定和染色方案。
