Department of Biochemistry and Cell Biology, Center for Developmental Genetics, Stony Brook University, Stony Brook, New York.
Dev Dyn. 2013 Nov;242(11):1320-31. doi: 10.1002/dvdy.24021. Epub 2013 Oct 7.
As the sea anemone Nematostella vectensis emerges as a model for studying regeneration, new tools will be needed to assess its regenerative processes and describe perturbations resulting from experimental investigation. Chief among these is the need for a universal set of staging criteria to establish morphological landmarks that will provide a common format for discussion among investigators.
We have established morphological criteria to describe stages for rapidly assessing regeneration of the aboral end (physa) of Nematostella. Using this staging system, we observed rates of regeneration that are temperature independent during wound healing and temperature dependent afterward. Treatment with 25 μM lipoic acid delays the progression through wound healing without significantly affecting the subsequent rate of regeneration. Also, while an 11-day starvation before amputation causes only a minimal delay in regeneration, this delay is exacerbated by lipoic acid treatment.
A system for staging the progression of regeneration in amputated Nematostella physa based on easily discernible morphological features provides a standard for the field. This system has allowed us to identify both temperature-dependent and -independent phases of regeneration, as well as a nutritional requirement for normal regenerative progression that is exacerbated by lipoic acid.
海葵 Nematostella vectensis 作为研究再生的模式生物而备受关注，因此需要新的工具来评估其再生过程并描述实验研究引起的干扰。其中最重要的是需要一套通用的分期标准，以确定形态学标志，为研究人员提供共同的讨论格式。
我们已经建立了描述海葵口冠（physa）再生的形态学标准。使用该分期系统，我们观察到在伤口愈合过程中，再生速度与温度无关，而之后则与温度有关。25 μM 硫辛酸处理可延迟伤口愈合过程，但对随后的再生速度无明显影响。此外，在截肢前 11 天饥饿仅会导致再生轻微延迟，但硫辛酸处理会加剧这种延迟。
基于易于识别的形态特征，建立了一种分期系统来评估海葵 physa 截肢后的再生进展，为该领域提供了一个标准。该系统使我们能够识别再生的温度依赖性和非依赖性阶段，以及正常再生进展所需的营养要求，而硫辛酸会加剧这种要求。
